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| Status |
Public on Jun 20, 2013 |
| Title |
Gene expression data in Meg-01 cells following transfection with platelet-enriched microRNA-1225-3p |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Platelets from sickle cell disease patients have differentially expressed microRNAs as compared to platelets from healthy control subjects. The objective of this study is to overexpress an upregulated miRNA,miR-1225-3p, in MEG01 cells to identify putative targets.
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| Overall design |
MEG-01 cells were transfected with microRNA-1225-3p mimic for overexpression. Simultaneously, a negative control (scrambled) RNA was transfected for comparison. Gene expression was measured at 24 hours after transfection. Five independent experiments were performed at the same time in each group: microRNA-1225-3p transfected and scrambled.
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| Contributor(s) |
Jain S, Gladwin MT, Kaminski N |
| Citation(s) |
23593351 |
| |
| Submission date |
Oct 15, 2012 |
| Last update date |
Oct 11, 2016 |
| Contact name |
Shilpa Jain |
| E-mail(s) |
shilpa.jain3@chp.edu
|
| Organization name |
Uinversity of Pittsburgh
|
| Street address |
200 Lothrop Street
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15213 |
| Country |
USA |
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| Platforms (1) |
| GPL14550 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Probe Name Version) |
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| Samples (10)
|
| GSM1019586 |
miR1225 transfected MEG-01, biological replicate 1 |
| GSM1019587 |
miR1225 transfected MEG-01, biological replicate 2 |
| GSM1019588 |
miR1225 transfected MEG-01, biological replicate 3 |
| GSM1019589 |
miR1225 transfected MEG-01, biological replicate 4 |
| GSM1019590 |
miR1225 transfected MEG-01, biological replicate 5 |
| GSM1019591 |
scrambled transfected MEG-01, biological replicate 1 |
| GSM1019592 |
scrambled transfected MEG-01, biological replicate 2 |
| GSM1019593 |
scrambled transfected MEG-01, biological replicate 3 |
| GSM1019594 |
scrambled transfected MEG-01, biological replicate 4 |
| GSM1019595 |
scrambled transfected MEG-01, biological replicate 5 |
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| This SubSeries is part of SuperSeries: |
| GSE41575 |
Role of platelet micrornas in sickle cell disease |
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| Relations |
| BioProject |
PRJNA177572 |
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