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| Status |
Public on Oct 19, 2012 |
| Title |
Modeling Human Cortical Development in vitro using induced Pluripotent Stem Cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Human iPSCs grown in suspension in the presence of rostral neuralizing factors generated three-dimensional structures containing polarized radial glia, intermediate progenitors and a spectrum of layer-specific cortical neurons reminiscent of their organization in vivo. The hiPSC-derived multilayered structures express a gene expression profile typical of the embryonic telencephalon, but not that of other CNS regions.
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| |
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| Overall design |
Total RNA obtained from iPSC lines and H1 line was submitted for microarray analysis using HumanHT-12 v4 BEADCHIP (Illumina).
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| |
|
| Contributor(s) |
Mariani J, Vaccarino FM |
| Citation(s) |
22761314 |
| |
| Submission date |
Oct 12, 2012 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Flora Vaccarino |
| Organization name |
Yale University
|
| Department |
Child Study Center
|
| Lab |
Vaccarino
|
| Street address |
230 South Frontage Road
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06520 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
|
| Samples (10)
|
| GSM1019377 |
iPSC PGP1-1 replicate 1 |
| GSM1019378 |
iPSC PGP1-1 replicate 2 |
| GSM1019379 |
neuronal differentiation at day 50 induced in low FGF for iPS 03-01#9 replicate 1 |
| GSM1019380 |
neuronal differentiation at day 50 induced in low FGF for iPS 03-01#9 replicate 2 |
| GSM1019381 |
neuronal differentiation at day 50 induced in high FGF for iPS 03-01#9 replicate 1 |
| GSM1019382 |
neuronal differentiation at day 50 induced in high FGF for iPS 03-01#9 replicate 2 |
| GSM1019383 |
hES H1 |
| GSM1019384 |
iPS #9 for individual 03-01 (2) |
| GSM1019385 |
neuronal differentiation at day 50 induced in low FGF for iPS PGP1-1 |
| GSM1019386 |
neuronal differentiation at day 50 induced in high FGF for iPS PGP1-1 |
|
| Relations |
| BioProject |
PRJNA177962 |
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