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| Status |
Public on Sep 06, 2012 |
| Title |
Expression data from E18.5 mouse dorsal telencephalon |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Radial progenitors deficient in both Mek1 and Mek2 fail to transition to the gliogenic mode in late embryogenesis, and astrocyte and oligodendroglial precursors fail to appear. In exploring mechanisms, we found the Ets transcription family member Etv5/Erm is strongly regulated by MEK. Our microarray assay showed that Erm is specifically downregulated in Mek mutant brain.
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| Overall design |
RNA was extracted from dorsal corticies of E18.5 WT or Mek1,2\NesCre conditional mutant embryos. There were totally 4 samples: 2 WT and 2 mutants. 200 ng of total RNA was prepared for microarray hybridization according to the GeneChip® Whole Transcript (WT) Sense Target Labeling Assay user manual.
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| Contributor(s) |
Li X, Newbern JM, Snider WD |
| Citation(s) |
22998872 |
| |
| Submission date |
Sep 06, 2012 |
| Last update date |
Mar 04, 2019 |
| Contact name |
xiaoyan li |
| E-mail(s) |
xiaoyan_li@med.unc.edu
|
| Organization name |
University of North Carolina
|
| Department |
Neuroscience Center
|
| Lab |
Dr. William Snider lab
|
| Street address |
115 Mason Farm Road
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (4)
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| GSM999081 |
control dorsal cortex_E18.5_rep1 |
| GSM999082 |
control dorsal cortex_E18.5_rep2 |
| GSM999083 |
Mek\NestinCre mutant dorsal cortex_E18.5_rep1 |
| GSM999084 |
Mek\NestinCre mutant dorsal cortex_E18.5_rep2 |
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| Relations |
| BioProject |
PRJNA174566 |
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