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Series GSE40654 Query DataSets for GSE40654
Status Public on Jan 01, 2016
Title Does Type of Dietary Fat Matter? Prostate Cancer Xenograft Progression in a SCID Mouse Model with Varying Dietary Fat Sources
Organism Homo sapiens
Experiment type Expression profiling by array
Summary PURPOSE: Previous mouse studies using corn oil (ω-6) as the dietary fat source suggest that decreasing dietary fat content can slow prostate cancer (PCa) growth. However, other studies, in which the diet was composed around saturated fat, showed no difference in outcomes between high-fat and low-fat diets. The relative effects of other fats, such as fish oil and olive oil, also remain unexplored. To our knowledge, no trial has yet compared the effect of various fats on prostate cancer progression. Therefore, we sought to systematically study the effect of fish oil, olive oil, corn oil, and saturated fat on prostate cancer progression. METHODS: A total of 96 male SCID mice were injected with LAPC-4 human PCa cells. Two weeks following injection, mice were singly-housed and randomized to either a fish oil, olive oil, corn oil, or saturated fat based diet. Animals were euthanized when tumors reached 1,000 mm3. Serum was collected at sacrifice and assayed for PSA, insulin, IGF-1, IGFBP-3, and PGE-2 levels. Tumors were also assayed for PGE-2, and COX-2 levels, and gene array analysis was performed. RESULTS: Mice weights and tumor volumes were equivalent across groups at randomization. Overall, fish-oil consumption was associated with improved survival, relative to all other dietary groups (Log-rank, all p<0.05). We did not detect any significant difference in serum PSA, insulin, IGF-1, IGFBP-3, and PGE-2 levels. Glucose at the time of sacrifice was statistically different between groups, with the fish-oil fed mice having the highest levels of serum glucose (Kruskal-Wallis, p=0.03).
CONCLUSIONS: In this prostate cancer xenograft model, we found that consuming a diet in which fish-oil was the only fat source slowed tumor growth in improved survival, compared to mice consuming diets composed of olive oil, corn oil, or saturated fat sources. These results suggest that type of dietary fat consumed may be as important as amount of dietary fat consumed in the setting of prostate cancer.
Overall design DESIGN: A total of 96 male SCID mice were injected with LAPC-4 human prostate cancer cells. After 2 weeks of tumor growth, the mice were randomized to one of four diets: corn oil, fish oil, olive oil, and saturated fat source diets. Animals were euthanized when tumor volumes exceeded 1000 mm^3. Sera and tissues from the median 6 surviving animals from each of the four dietary groups were analyzed.
Contributor(s) Lloyd JC, Masko EM, Wu C, Melissa K, Aronson WJ, Chi JA, Freedland SJ
Citation(s) 23877027
Submission date Sep 06, 2012
Last update date Dec 06, 2018
Contact name Jen-Tsan Chi
Phone 919-668-4759
Organization name Duke University
Department Molecular Genetics and Microbiology
Street address 101 Science Drive, DUMC 3382
City Durham
State/province NC
ZIP/Postal code 27708
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (24)
GSM998894 Corn oil diet 1
GSM998895 Corn oil diet 2
GSM998896 Corn oil diet 3
BioProject PRJNA174516

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Supplementary file Size Download File type/resource
GSE40654_RAW.tar 44.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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