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| Status |
Public on Dec 10, 2012 |
| Title |
Effects of a 8-week training on human skeletal muscle |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Context: Exercise training is a plausible model for identification of molecular mechanisms that cause metabolic-related changes in human skeletal muscle.
Objective: The goal was to explore the molecular basis of the adaptation of skeletal muscle to exercise training.
Design and Intervention: Obese male subjects were subjected to an individualized supervised training program targeted in order to optimize lipid oxidation during 8 weeks.
Main Outcome Measures: Primary outcome measures were gene expression profiling of skeletal muscle. Body composition, oral glucose tolerance test, Resting metabolic rate, respiratory quotient, maximal oxygen uptake and metabolic biochemistry were also assessed.
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| Overall design |
Overall Design
The obese (BMI 30-36) male volunteers (age 32-42) were asked to refrain from vigorous physical activity 48h before presenting to the clinical investigation centre, and ate a weight-maintaining diet consisting of 35% fat, 16% protein, and 49% carbohydrates two days before the experiment. Muscle biopsies of Vastus Lateralis weighing 60–100 mg were obtained using the Bergstrom technique, cleaned and snap-frozen in liquid nitrogen. Resting metabolic rate, respiratory quotient and maximal oxygen uptake were assessed. The subjects were investigated at baseline and after 8 weeks of supervised aerobic exercise training program consisting of daily sessions of 45-60 min of endurance exercise, 5 days a week, at least 48-72h after the last acute exercise bout. Skeletal muscle biopsies were obtained at the beginning and at the end of the protocol. Transcriptome analysis compared 8 subjects before vs. after training using arrays using a common reference design (Cy5 dye was incorporated into all muscle RNA samples, while a reference RNA pool made of the mix of commercial human liver, adipose tissue and skeletal muscle RNA was labelled with Cy3 dye (Applied Biosystems/Ambion, Foster City, USA)( and whole genome 4x44k oligonucleotide arrays (Agilent Technologies).
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| Contributor(s) |
Viguerie N, Montastier E, Besse-Patin A, Combes M, Moro C, Langin D |
| Citation(s) |
23114600 |
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| Submission date |
Sep 03, 2012 |
| Last update date |
Dec 11, 2012 |
| Contact name |
Nathalie Viguerie |
| Organization name |
INSERM
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| Department |
I2MC U1297
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| Lab |
MetaDiab
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| Street address |
Avenue Poulhes
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| City |
Toulouse |
| ZIP/Postal code |
31400 |
| Country |
France |
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| Platforms (1) |
| GPL16022 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Gene Symbol Version) |
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| Samples (16)
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| GSM994975 |
Skeletal muscle from obese male 18, control |
| GSM995422 |
Skeletal muscle from obese male 18 after 8-week training |
| GSM995435 |
Skeletal muscle from obese male 3, control |
| GSM995542 |
Skeletal muscle from obese male 3 after 8-week training |
| GSM995703 |
Skeletal muscle from obese male 8, control |
| GSM995704 |
Skeletal muscle from obese male 1, control |
| GSM995705 |
Skeletal muscle from obese male 16, control |
| GSM995706 |
Skeletal muscle from obese male 25, control |
| GSM995711 |
Skeletal muscle from obese male 20, control |
| GSM995712 |
Skeletal muscle from obese male 8 after 8-week training |
| GSM995774 |
Skeletal muscle from obese male 1 after 8-week training |
| GSM995775 |
Skeletal muscle from obese male 16 after 8-week training |
| GSM995776 |
Skeletal muscle from obese male 17 after 8-week training |
| GSM995927 |
Skeletal muscle from obese male 25 after 8-week training |
| GSM995928 |
Skeletal muscle from obese male 20 after 8-week training |
| GSM995929 |
Skeletal muscle from obese male 17, control |
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| Relations |
| BioProject |
PRJNA174449 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE40551_RAW.tar |
178.5 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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