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| Status |
Public on Aug 01, 2013 |
| Title |
Genome-wide GTL1 binding sites in Arabidopsis thaliana |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
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| Summary |
To understand how GTL1 regulates cell growth, we first identified its potential direct targets by the chromatin immunoprecipitation followed by the hybridization on an Affymetrix Arabidopsis Tiling 1.0R array (ChIP-chip). To enrich the genomic region bound by GTL1 in vivo, we harvested whole aerial parts of 12-day-old gtl1-1 plants complemented with the pGTL:GTL1:GFP constructs and immunoprecipitated the chromatin fragments associated with GTL1-GFP proteins using antibodies against GFP. After applying a cut-off P-values of 0.001of MAT (Model-based analysis of tiling array), we identified a total number of 3,900 putative immediate target genes that showed consistent binding by GTL1.
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| Overall design |
Two IP chips compared to two Input chips.
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| Contributor(s) |
Breuer C, Morohashi K, Grotewold E, Sugimoto K |
| Citation(s) |
23143274 |
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| Submission date |
Aug 31, 2012 |
| Last update date |
Sep 04, 2019 |
| Contact name |
Kengo Morohashi |
| E-mail(s) |
morohashi.1@osu.edu
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| Phone |
614-688-4954
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| Organization name |
The Ohio State University
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| Department |
Molecular Genetics
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| Lab |
Erich Grotewold
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| Street address |
1060 Carmack Rd
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| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43210 |
| Country |
USA |
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| Platforms (1) |
| GPL10977 |
[At35b_MR] Affymetrix Arabidopsis Tiling 1.0R Array |
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| Samples (1) |
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| This SubSeries is part of SuperSeries: |
| GSE40520 |
GTL1 regulates ploidy-dependent cell growth through direct transcriptional repression of APC/CCS52A1 in Arabidopsis |
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| Relations |
| BioProject |
PRJNA174210 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE40519_RAW.tar |
156.3 Mb |
(http)(custom) |
TAR (of BAR, BED, CEL, TXT) |
| Processed data provided as supplementary file |
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