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Status |
Public on Jan 08, 2013 |
Title |
Gene expression analysis of Ncor1 muscle-specific knockout and PGC-1alpha muscle-specific transgenic skeletal muscle |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
In the present study we have studied the mechanistic and functional aspects of NCoR1 function in mouse skeletal muscle. NCoR1 muscle-specific knockout mice exhibited an increased oxidative metabolism. Global gene expression analysis revealed a high overlap between the effects of NCoR1 deletion and peroxisome proliferator-activated receptor (PPAR) gamma coactivator 1alpha (PGC-1alpha) overexpression on oxidative metabolism in skeletal muscle. The repressive effect of NCoR1 on oxidative phosphorylation gene expression specifically antagonizes PGC-1alpha-mediated coactivation of ERRalpha. We therefore delineated the molecular mechanism by which a transcriptional network controlled by corepressor and coactivator proteins determines the metabolic properties of skeletal muscle, thus representing a potential therapeutic target for metabolic diseases.
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Overall design |
Gene expression of a total of 20 gastrocnemius samples from control (CON, n = 5), NCoR1 muscle-specific knockout (NCoR1 MKO, n = 5), wild type (WT, n = 5) and PGC-1alpha muscle-specific transgenic (PGC-1alpha mTg, n = 5) adult male mice was analyzed using GeneChip® Gene 1.0 ST Array System (Affymetrix). NCoR1 MKO and PGC-1alpha mTg samples were compared to CON and WT samples, respectively.
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Contributor(s) |
Handschin C |
Citation(s) |
23028049 |
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Submission date |
Aug 28, 2012 |
Last update date |
Mar 04, 2019 |
Contact name |
Joaquín Pérez-Schindler |
E-mail(s) |
j.perezschindler@unibas.ch
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Organization name |
University of Basel
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Department |
Biozentrum
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Street address |
Klingelbergstrasse 50 / 70
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City |
Basel |
ZIP/Postal code |
4056 |
Country |
Switzerland |
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Platforms (1) |
GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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Samples (20)
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Relations |
BioProject |
PRJNA174100 |