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| Status |
Public on Sep 01, 2015 |
| Title |
RNASeq analysis of Salmonella B cell infection model |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We report the transcriptome analysis of S. enterica 14028 during infection of murine B cells. Mice spleens were infected with Salmonella enterica 14028. Mouse spleens were harvested and cells FACS sorted into various immune cells containing bacteria. Short reads were mapped to the S. enterica 14028 genome.
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| Overall design |
Mice were infected with S. enterica strain 14028. Spleens were collected, homogenized and cells FACS sorted. Total RNA was extracted using the Ambion mirVana RNA isolation kit. Purified RNA was depleted of rRNA utilizing the epicentre ribozero metabacteria and eukaryotic kits.
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| Contributor(s) |
Fred H, Hyunjin Y, Marcus J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 17, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Marcus Jones |
| E-mail(s) |
mjones@jcvi.org
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| Organization name |
JCVI
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| Street address |
4120 Torrey Pines Road
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (1) |
| GSM988446 |
S. enterica transcriptome analysis in mouse B cells FACS sorted from the spleen |
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| Relations |
| BioProject |
PRJNA173093 |
| SRA |
SRP014913 |
