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Series GSE40211 Query DataSets for GSE40211
Status Public on Sep 01, 2015
Title RNASeq analysis of Salmonella B cell infection model
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We report the transcriptome analysis of S. enterica 14028 during infection of murine B cells. Mice spleens were infected with Salmonella enterica 14028. Mouse spleens were harvested and cells FACS sorted into various immune cells containing bacteria. Short reads were mapped to the S. enterica 14028 genome.
Overall design Mice were infected with S. enterica strain 14028. Spleens were collected, homogenized and cells FACS sorted. Total RNA was extracted using the Ambion mirVana RNA isolation kit. Purified RNA was depleted of rRNA utilizing the epicentre ribozero metabacteria and eukaryotic kits.
Contributor(s) Fred H, Hyunjin Y, Marcus J
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Submission date Aug 17, 2012
Last update date May 15, 2019
Contact name Marcus Jones
Organization name JCVI
Street address 4120 Torrey Pines Road
City San Diego
State/province CA
ZIP/Postal code 92037
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (1)
GSM988446 S. enterica transcriptome analysis in mouse B cells FACS sorted from the spleen
BioProject PRJNA173093
SRA SRP014913

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE40211_RAW.tar 90.0 Kb (http)(custom) TAR (of TXT)
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Processed data provided as supplementary file
Raw data are available in SRA

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