|
| Status |
Public on Jan 05, 2006 |
| Title |
Contribution of unique genes by individual cell lines to Universal Human Reference RNA |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Total RNA isolated from 10 individual human cell lines were reverse-transcribed to cDNA, labeled with Cy5 and co-hybridized with Cy3-labeled UHRR onto 43,000-spot cDNA microarrays (Stanford University). The data was analyzed using GeneTraffic software. Approximately 6000-8000 spots out of 43,000 (14-18 %) were flagged on each microarray and excluded from further analysis. Spots with hybridization signals in Cy5 channel higher than 1000 and with Cy5/Cy3 ratio greater than 2 were collected and the number of spots with these characteristics in only one cell line was determined.
A reference experiment design type is where all samples are compared to a common reference.
Keywords: reference_design
|
| |
|
| Overall design |
Computed
|
| |
|
| Contributor(s) |
Novoradovskaya N |
| Citation(s) |
15113400 |
| |
| Submission date |
Jan 04, 2006 |
| Last update date |
Mar 17, 2012 |
| Organization |
Stanford Microarray Database (SMD) |
| E-mail(s) |
array@genome.stanford.edu
|
| Phone |
650-498-6012
|
| URL |
http://genome-www5.stanford.edu/
|
| Department |
Stanford University, School of Medicine
|
| Street address |
300 Pasteur Drive
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platforms (1) |
|
| Samples (10)
|
|
| This SubSeries is part of SuperSeries: |
| GSE4389 |
Universal Reference RNA as a standard for microarray experiments |
|
| Relations |
| BioProject |
PRJNA104875 |
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