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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 30, 2015 |
| Title |
Gene expression profiles of adult neural stem cells with loss-of-function and gain-of-function of SIRT1 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To elucidate the role of SIRT1 in adult neural stem cells, we have carried out a genome-wide microarray analysis on cultured adult neurospheres from wildtype (WT) and sirt1 knockout (KO) mice and WT neurospheres treated with DMSO and SIRT1 activator,resveratrol for 24 hours. Then we screened for genes that exhibited opposite changing patterns beween KO-versus-WT and resveratrol-versus-DMSO-treated groups. Our result revealed a significant enrichment of genes involved in metabolic process. And numerous genes involved in cell proliferation and differentiation were represented in these SIRT1-responsive genes. More interestingly, Notch signaling-related genes,such as Dll4, Mib1,Hes5, Heyl, Hey2 and so on, also showed a significant enrichment. Real-time PCR validated the expression of Notch signaling-related genes. These data revealed the regulation of SIRT1 on the self-renewal and differentiation of adult neural stem cells.
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| Overall design |
Secondary neurospheres from WT and sirt1 KO adult mice, and WT neurospheres treated with DMSO and resveratrol for 24 hours were used, each in duplicate.
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| Contributor(s) |
Xiaobing Y, Chenyan M |
| Citation(s) |
25468938 |
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| Submission date |
Jul 21, 2012 |
| Last update date |
Jan 12, 2017 |
| Contact name |
Chenyan Ma |
| Organization name |
UC Berkeley
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| Street address |
230E Li Ka Shing Center
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| City |
Berkeley |
| State/province |
CA |
| ZIP/Postal code |
94720 |
| Country |
USA |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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| Samples (8)
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| GSM971595 |
sirt1_KO 2nd_neurosphere _6DIV_rep2 |
| GSM971596 |
WT_2nd_neurosphere _24h_DMSO_rep1 |
| GSM971597 |
WT_2nd_neurosphere _24h_resveratrol_rep1 |
| GSM971598 |
WT_2nd_neurosphere _24h_DMSO_rep2 |
| GSM971599 |
WT_2nd_neurosphere _24h_resveratrol_rep2 |
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| Relations |
| BioProject |
PRJNA171112 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE39551_RAW.tar |
17.5 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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