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| Status |
Public on Jul 01, 2014 |
| Title |
Expression data from Saccharomyces cerevisiae WT, WT(hsf1), hac1∆ and hac1∆(hsf1) |
| Platform organisms |
Schizosaccharomyces pombe; Saccharomyces cerevisiae |
| Sample organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
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| Summary |
In the yeast Saccharomyces cerevisiae, accumulation of misfolded proteins in the endoplasmic reticulum (ER) causes ER stress and activates the unfolded protein response (UPR) mediated by Hac1p, whereas the heat shock response (HSR) mediated by Hsf1p mainly regulates cytosolic processes and protects the cell from different stresses. In this study, we find that a constitutive activation of the HSR by over-expression of a mutant HSF1 gene could relieve ER stress in both wild type and hac1∆ UPR-deficient cells.
We studied the genome-wide transcriptional response in order to identify regulatory mechanisms that govern the interplay between UPR and HSR responses. Interestingly, we find that the regulation of ER stress via HSR is mainly through facilitation of protein folding and secretion and not via the induction of Rpn4-dependent proteasomal activity.
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| Overall design |
Four Saccharomyces cerevisiae strains, WT, WT(hsf1), hac1∆ and hac1∆(hsf1), were grown in SD-URA medium and treated with 2.5 mM DTT. After two hours induction, samples were taken for RNA extraction and hybridization on Affymetrix microarrays. Biological triplicates were applied.
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| Contributor(s) |
Hou J, Österlund T |
| Citation(s) |
24237754 |
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| Submission date |
Jul 12, 2012 |
| Last update date |
Feb 21, 2017 |
| Contact name |
Tobias Österlund |
| E-mail(s) |
tobiaso@chalmers.se
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| Organization name |
Chalmers University of Technology
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| Department |
Dept. of Chemical and Biological Engineering
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| Lab |
Systems biology
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| Street address |
Kemivägen 10
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| City |
Göteborg |
| ZIP/Postal code |
41296 |
| Country |
Sweden |
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| Platforms (1) |
| GPL2529 |
[Yeast_2] Affymetrix Yeast Genome 2.0 Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA170538 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE39311_RAW.tar |
9.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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