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| Status |
Public on Jul 13, 2012 |
| Title |
Identification of genes responsive to mild hyperthermia in human normal fibroblast OUMS-36 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Hyperthermia is widely used to treat patients with various cancers. Here, the effects of heat stress at 41°C for 30 min (mild hyperthermia) on the gene expression in OUMS-36 human normal fibroblast cells were investigated using an Affymetrix GeneChip system. The cells were treated with mild hyperthermia, followed by incubation for 0, 1, or 3 h at 37°C. No cell death was observed in the mild hyperthermia-treated cells. On the other hand, many genes that were differentially expressed by a factor 1.5 or greater were identified in the cells treated with the mild hyperthermia.
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| Overall design |
OUMS-36 human normal fibroblast cells were treated with heat stress (41°C for 30 min), followed by incubation for 0, 1, or 3 h at 37°C. Non-treated cells served as the control. Total RNA samples were prepared from the cells, and quality of the RNA was analyzed using a Bioanalyzer 2100. Gene expression was monitored by an Affymetrix GeneChip® system with a Human Genome U133-plus 2.0 array. Sample preparation for array hybridization was carried out as described in the manufacturer's instructions.
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| Contributor(s) |
Tabuchi Y, Kariya A, Furusawa Y |
| Citation(s) |
23311377 |
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| Submission date |
Jul 07, 2012 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Yoshiaki Tabuchi |
| E-mail(s) |
ytabu@cts.u-toyama.ac.jp
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| Organization name |
University of Toyama
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| Department |
Life Science Research Center
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| Lab |
Division of Molecular Genetics Research
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| Street address |
2630 Sugitani
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| City |
Toyama |
| State/province |
Toyama |
| ZIP/Postal code |
930-0194 |
| Country |
Japan |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (4)
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| GSM957602 |
OUMS-36 cells (control) |
| GSM957603 |
OUMS-36 cells treated with heat stress at 41°C and cultured for 0 h |
| GSM957604 |
OUMS-36 cells treated with heat stress at 41°C and cultured for 1 h |
| GSM957605 |
OUMS-36 cells treated with heat stress at 41°C and cultured for 3 h |
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| Relations |
| BioProject |
PRJNA170530 |
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