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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Nov 29, 2012 |
| Title |
Mammalian cells acquire epigenetic hallmarks of human cancer during immortalization |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by genome tiling array
Expression profiling by array
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| Summary |
Progression to malignancy requires cells to overcome senescence and switch to an immortal phenotype. Thus, exploring the genetic and epigenetic changes that occur during senescence/immortalization may help elucidate crucial events that lead to cell transformation. In the present study, we have globally profiled DNA methylation in relation to gene expression in primary, senescent and immortalized mouse embryonic fibroblasts.
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| Overall design |
Primary, senescent and immortalized mouse fibroblasts obtained from three independent embryos. Compare immortalized versus primary and senescent versus primary cells.
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| Contributor(s) |
Tommasi S, Zheng A, Weninger A, Bates SE, Li AX, Wu X, Hollstein M, Besaratinia A |
| Citation(s) |
23143272 |
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| Submission date |
Jun 29, 2012 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Xiwei Wu |
| E-mail(s) |
xwu@coh.org
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| Organization name |
City of Hope National Medical Center
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| Department |
Computational and Quantitative Medicine
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| Street address |
1500 E. Duarte Rd.
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| City |
Duarte |
| State/province |
CA |
| ZIP/Postal code |
91010 |
| Country |
USA |
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| Platforms (2) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
| GPL15761 |
NimbleGen mouse DNA Methylation 3x720K CpG Island Plus RefSeq Promoter Arrays |
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| Samples (17)
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| GSM954262 |
Mouse embryonic fibroblasts. Set 1, primary, passage # <6 (methylation) |
| GSM954263 |
Mouse embryonic fibroblasts. Set 1, immortalized, passage # >10 (methylation) |
| GSM954264 |
Mouse embryonic fibroblasts. Set 2, primary, passage # <6 (methylation) |
| GSM954265 |
Mouse embryonic fibroblasts. Set 2, senescent, passage # 6-10 (methylation) |
| GSM954266 |
Mouse embryonic fibroblasts. Set 2, immortalized, passage # >10 (methylation) |
| GSM954267 |
Mouse embryonic fibroblasts. Set 3, primary, passage # <6 (methylation) |
| GSM954268 |
Mouse embryonic fibroblasts. Set 3, senescent, passage # 6-10 (methylation) |
| GSM954269 |
Mouse embryonic fibroblasts. Set 3, immortalized, passage # >10 (methylation) |
| GSM954270 |
Mouse embryonic fibroblasts, primary, passage # <6 Mo.21 (expression) |
| GSM954271 |
Mouse embryonic fibroblasts, senescent, passage # 6-10 Mo.22 (expression) |
| GSM954272 |
Mouse embryonic fibroblasts, immortalized, passage # >10 Mo.23 (expression) |
| GSM954273 |
Mouse embryonic fibroblasts, primary, passage # <6 Mo.24 (expression) |
| GSM954274 |
Mouse embryonic fibroblasts, senescent, passage # 6-10 Mo.25 (expression) |
| GSM954275 |
Mouse embryonic fibroblasts, immortalized, passage # >10 Mo.26 (expression) |
| GSM954276 |
Mouse embryonic fibroblasts, primary, passage # <6 Mo.27 (expression) |
| GSM954277 |
Mouse embryonic fibroblasts, senescent, passage # 6-10 Mo.28 (expression) |
| GSM954278 |
Mouse embryonic fibroblasts, immortalized, passage # >10 Mo.29 (expression) |
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| Relations |
| BioProject |
PRJNA169641 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE39034_RAW.tar |
406.7 Mb |
(http)(custom) |
TAR (of CEL, CHP, GFF, PAIR) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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