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| Status |
Public on Jan 24, 2014 |
| Title |
ChIP-seq of bovine cells (MDBK) to study butyrate-induced histone modification |
| Organism |
Bos taurus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Utilizing next-generation sequencing technology, combined with ChIP (Chromatin Immunoprecipitation) technology to analyze histone modification (acetylation) induced by butyrate and to map the epigenomic landscape of normal histone H3, H4
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| |
|
| Overall design |
Cells were treated with 10 mM butyrate for 24 hr, The cells were scraped from the flask and homogenized with ice-cold Dounce homogenizer to release the nuclei. The collected nuclei were resuspended in digestion buffer and enzymatic shearing was performed. ChIP with anti H3, H4 and acetyl-H3 and acetyl-H4.
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| |
|
| Contributor(s) |
Li C, Li R |
| Citation(s) |
23645985 |
| |
| Submission date |
Jun 27, 2012 |
| Last update date |
Oct 28, 2019 |
| Contact name |
Congjun Li |
| E-mail(s) |
congjun.li@ars.usda.gov
|
| Organization name |
ARS, USDA
|
| Department |
BFGL
|
| Street address |
10300 Baltimore Ave
|
| City |
Beltsville |
| State/province |
MD |
| ZIP/Postal code |
20705 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL15749 |
Illumina HiSeq 2000 (Bos taurus) |
| GPL15750 |
Illumina Genome Analyzer IIx (Bos taurus) |
|
| Samples (10)
|
|
| Relations |
| BioProject |
PRJNA169504 |
| SRA |
SRP013959 |
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