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Series GSE38973 Query DataSets for GSE38973
Status Public on Jan 24, 2014
Title ChIP-seq of bovine cells (MDBK) to study butyrate-induced histone modification
Organism Bos taurus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Utilizing next-generation sequencing technology, combined with ChIP (Chromatin Immunoprecipitation) technology to analyze histone modification (acetylation) induced by butyrate and to map the epigenomic landscape of normal histone H3, H4
Overall design Cells were treated with 10 mM butyrate for 24 hr, The cells were scraped from the flask and homogenized with ice-cold Dounce homogenizer to release the nuclei. The collected nuclei were resuspended in digestion buffer and enzymatic shearing was performed. ChIP with anti H3, H4 and acetyl-H3 and acetyl-H4.
Contributor(s) Li C, Li R
Citation(s) 23645985
Submission date Jun 27, 2012
Last update date Oct 28, 2019
Contact name Congjun Li
Organization name ARS, USDA
Department BFGL
Street address 10300 Baltimore Ave
City Beltsville
State/province MD
ZIP/Postal code 20705
Country USA
Platforms (2)
GPL15749 Illumina HiSeq 2000 (Bos taurus)
GPL15750 Illumina Genome Analyzer IIx (Bos taurus)
Samples (10)
GSM953078 acetyl-H3K18-ChIP-seq
GSM953079 trimethyl-H3K9-ChIP-seq
GSM953080 Histone H4 ChIP-seq
BioProject PRJNA169504
SRA SRP013959

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Supplementary file Size Download File type/resource
GSE38973_RAW.tar 2.7 Gb (http)(custom) TAR (of TAR)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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