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Series GSE38959 Query DataSets for GSE38959
Status Public on Dec 21, 2012
Title Gene expression profiling of triple negative breast cancer, normal ductal cells, and normal tissues
Organism Homo sapiens
Experiment type Expression profiling by array
Summary To identify novel molecular targets for triple negative breast cancer (TNBC), we have employed whole genome microarray expression profiling. We purified 30 surgically resected breast cancer tissue diagnosed triple negative by means of immunohistochemical staining and 13 normal mammary ductal cells with lasermicrobeam microdissection system (PALM MicroBeam, Carl Zeiss MicroImaging Co., Ltd), performed whole human genome microarray, and compared gene expression levels of TNBC, normal mammary ductal cells, and normal vital organs to develop molecular targets with a minimum risk.
 
Overall design Gene expression levels of 30 TNBC, 13 normal mammary ductal cells, and 4 normal vital tissues were evaluated. to clarify the molecular mechanism involved in TNBC, we analyzed gene expression profiles of 30 TNBC as well as 13 normal epithelial ductal cells purified by laser microbeam microdissection, and identified 301 transcripts that were significantly up-regulated and 321 transcripts that were significantly down-regulated in TNBC. In addition, gene-expression profiles analysis of normal human vital organs including heart, lung, liver, and kidney allowed us to identify 90 cancer-specific genes involved in breast carcinogenesis such as NEK2, PBK, DTL, MELK and GPSM2. Among them, we focused on cell cycle regulators, asp (abnormal spindle) homolog, microcephaly associated (Drosophila) (ASPM) and centromere protein K (CENPK) as novel therapeutic targets for TNBC.
 
Contributor(s) Komatsu M, Katagiri T
Citation(s) 23254957
Submission date Jun 27, 2012
Last update date Feb 22, 2018
Contact name Masato Komatsu
E-mail(s) komatsu@genome.tokushima-u.ac.jp
Phone 0886339476
Organization name Genome Medicine
Street address Kuramoto-cho 3-18-15
City Tokushima
ZIP/Postal code 7708503
Country Japan
 
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (47)
GSM952871 triple negative breast cancer cells T253
GSM952872 triple negative breast cancer cells T359
GSM952873 triple negative breast cancer cells T362
Relations
BioProject PRJNA169423

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE38959_RAW.tar 96.9 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Processed data provided as supplementary file
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