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Series GSE38958 Query DataSets for GSE38958
Status Public on Jun 30, 2014
Title Profiling of Gene Expression in Idiopathic Pulmonary Fibrosis
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Idiopathic pulmonary fibrosis (IPF) is a specific form of chronic, progressive fibrosing interstitial disease of unknown cause. It remains impractical to conduct early diagnosis and predict IPF progression just based on gene expression information. Moreover, the relationship between gene expression and quantitative phenotypic value in IPF keeps controversial. To identify biomarkers to predict survival in IPF, we profiled protein-coding gene expression in peripheral blood mononuclear cells (PBMCs). We linked the gene expression level with the quantitative phenotypic variation in IPF, including diffusing capacity of the lung for carbon monoxide (DLCO) and forced vital capacity (FVC) percent predicted. In silico analyses on the expression profiles and quantitative phenotypic data allowed for the generation of a set of IPF molecular signature that predicted survival of IPF effectively.
 
Overall design Total RNA was isolated from PBMCs using standard molecular biology protocols without DNA contamination or RNA degradation. Sample processing (e.g., cDNA generation, fragmentation, end labeling, hybridization to Affymetrix GeneChip Human Exon 1.0 ST arrays) was performed per manufacturer’s instructions. A total of 45 healthy controls and 70 IPF patients were included in the microarray analysis.
 
Contributor(s) Zhou T, Zhang W, Ma SF, Wade M, Noth I, Garcia JG
Citation(s) 26286721, 24779708
Submission date Jun 27, 2012
Last update date Oct 22, 2019
Contact name Tong Zhou
Organization name University of Nevada School of Medicine
Street address 1664 N Virginia St
City Reno
State/province NV
ZIP/Postal code 89557
Country USA
 
Platforms (1)
GPL5175 [HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version]
Samples (115)
GSM952756 Healthy control C1
GSM952757 Healthy control C2
GSM952758 Healthy control C3
Relations
BioProject PRJNA169421

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE38958_RAW.tar 2.5 Gb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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