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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Sep 01, 2012 |
| Title |
Epstein-Barr virus LMP1 and LMP2A transgenic B lymphocytes and lymphoma cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Gene expression profile of splenic B cells (CD19+) from transgenic mice expressing the Epstein-Barr virus (EBV) latent membrane proteins (LMP) 1 and/or LMP2A.
Freshly harvested primary B cells were profiled. B lymphocytes from transgenic LMP1, LMP2A, LMP1/2A mice and negative littermates were profiled from 6 month old adult mice; lymphoma cells were passaged in SCID mice and profiled for three LMP1 positive lymphomas and one negative lymphoma.
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| Overall design |
12 total samples. 4 transgenic B lymphocyte samples pooled from multiple biological replicates were hybridized to duplicate microarrays: LMP1 (pooled from 2 replicates), LMP2A (pooled from 3 replicates); LMP1/2A (pooled from 5 replicates), negative littermates (pooled from 4 replicates). 3 biological replicates of LMP1 lymphomas expressing high, medium and low levels of LMP1 and; 1 negative lymphoma was hybridized to 1 microarray chip. The reference sample consisted of 4 biological replicates of splenic B cells (CD19+) pooled from 4-7 month old non-transgenic Balb/c mice. The same reference was used for all hybridizations.
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| Contributor(s) |
Shair KH, Raab-Traub N |
| Citation(s) |
22991431 |
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| Submission date |
Jun 26, 2012 |
| Last update date |
Jan 12, 2017 |
| Contact name |
Kathy Shair |
| Organization name |
University of Pittsburgh
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| Department |
UPMC Hillman Cancer Center
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| Lab |
1.8
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| Street address |
5117 Centre Avenue
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| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15213 |
| Country |
USA |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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| Samples (12)
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| GSM952718 |
negative littermate lymphocyte, experimental replicate 1 |
| GSM952719 |
LMP1 lymphocyte, experimental replicate 1 |
| GSM952720 |
LMP2A lymphocyte, experimental replicate 1 |
| GSM952721 |
LMP1/2A lymphocyte, experimental replicate 1 |
| GSM952722 |
negative littermate lymphocyte, experimental replicate 2 |
| GSM952723 |
LMP1 lymphocyte, experimental replicate 2 |
| GSM952724 |
LMP2A lymphocyte, experimental replicate 2 |
| GSM952725 |
LMP1/2A lymphocyte, experimental replicate 2 |
| GSM952726 |
Negative lymphoma cell |
| GSM952727 |
LMP1 lymphoma cell-high expression |
| GSM952728 |
LMP1 lymphoma cell-medium expression |
| GSM952729 |
LMP1 lymphoma cell-low expression |
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| Relations |
| BioProject |
PRJNA169564 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE38954_RAW.tar |
170.8 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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