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Series GSE38954 Query DataSets for GSE38954
Status Public on Sep 01, 2012
Title Epstein-Barr virus LMP1 and LMP2A transgenic B lymphocytes and lymphoma cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary Gene expression profile of splenic B cells (CD19+) from transgenic mice expressing the Epstein-Barr virus (EBV) latent membrane proteins (LMP) 1 and/or LMP2A.
Freshly harvested primary B cells were profiled. B lymphocytes from transgenic LMP1, LMP2A, LMP1/2A mice and negative littermates were profiled from 6 month old adult mice; lymphoma cells were passaged in SCID mice and profiled for three LMP1 positive lymphomas and one negative lymphoma.
Overall design 12 total samples. 4 transgenic B lymphocyte samples pooled from multiple biological replicates were hybridized to duplicate microarrays: LMP1 (pooled from 2 replicates), LMP2A (pooled from 3 replicates); LMP1/2A (pooled from 5 replicates), negative littermates (pooled from 4 replicates). 3 biological replicates of LMP1 lymphomas expressing high, medium and low levels of LMP1 and; 1 negative lymphoma was hybridized to 1 microarray chip. The reference sample consisted of 4 biological replicates of splenic B cells (CD19+) pooled from 4-7 month old non-transgenic Balb/c mice. The same reference was used for all hybridizations.
Contributor(s) Shair KH, Raab-Traub N
Citation(s) 22991431
Submission date Jun 26, 2012
Last update date Jan 12, 2017
Contact name Kathy Shair
Organization name University of Pittsburgh
Department UPMC Hillman Cancer Center
Lab 1.8
Street address 5117 Centre Avenue
City Pittsburgh
State/province PA
ZIP/Postal code 15213
Country USA
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (12)
GSM952718 negative littermate lymphocyte, experimental replicate 1
GSM952719 LMP1 lymphocyte, experimental replicate 1
GSM952720 LMP2A lymphocyte, experimental replicate 1
BioProject PRJNA169564

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Supplementary file Size Download File type/resource
GSE38954_RAW.tar 170.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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