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| Status |
Public on Jan 24, 2013 |
| Title |
Analysis of TF binding sites and histone modification in IRF8-induced monocyte differentiation |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Chromatins were prepared from myeloid progenitor cell line Tot2 cells transduced with retrovirus for control MSCV or MSCV-IRF8. Chromatin immunoprecipitation (ChIP) was carried out by using anti-IRF8 antibody, anti-PU.1 antibody or anti-histone H3K4me1 antibody.
ChIP-Seq peaks were identified using the FindPeaks tool in the HOMER package or SICER with input DNA tags as background distribution.
Potential IRF8-direct target genes were identified based on ChIP-Seq and the alteration of expression by IRF8-induction.
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| Overall design |
Examination of 2 transcription factor occupancy and 1 histone modifications in myeloid progenitor cells and monocyte-like cells differetiated by IRF8 expression
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| Contributor(s) |
Nishiyama A, Tamura T |
| Citation(s) |
23319570 |
| Submission date |
Jun 20, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Tomohiko Tamura |
| E-mail(s) |
tamurat@yokohama-cu.ac.jp
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| Organization name |
Yokohama City University
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| Department |
Department of Immunology
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| Street address |
3-9 Fukuura, Kanazawa-ku
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| City |
Yokohama |
| ZIP/Postal code |
236-0004 |
| Country |
Japan |
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| Platforms (1) |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE38825 |
IRF8-induced monocyte differentiation |
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| Relations |
| BioProject |
PRJNA169037 |
| SRA |
SRP013833 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE38824_RAW.tar |
402.6 Mb |
(http)(custom) |
TAR (of BEDGRAPH) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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