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Status |
Public on Sep 14, 2012 |
Title |
The maize pericarp transcriptome |
Organism |
Zea mays |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
P1 encodes an R2R3-MYB transcription factor responsible for the accumulation of insecticidal flavones in maize silks and red phlobaphene pigments in pericarps and other floral tissues, which contributed to making P1 an important visual marker since the dawn of modern genetics. We conducted RNA-Seq using pericarps at two different stages, 14 and 25 days after pollination (DAP). High-throughput sequencing using the Illumina platform resulted in the generation of ~20 million high quality reads, from which ~90% aligned to the recently completed maize genome sequence corresponding to ~5 million reads for each one of the four samples.
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Overall design |
Examination of two different RNA samples from two different stages of maize pericarp tissues.
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Contributor(s) |
Morohashi K, Mejia-Guerr MK, Casas MI, Grotewold E |
Citation(s) |
22822204 |
Submission date |
Jun 01, 2012 |
Last update date |
May 15, 2019 |
Contact name |
Erich Grotewold |
Organization name |
Michigan State University
|
Lab |
Grotewold Laboratory
|
Street address |
603 Wilson Road
|
City |
East Lansing |
State/province |
MI |
ZIP/Postal code |
48823 |
Country |
USA |
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Platforms (1) |
GPL9361 |
Illumina Genome Analyzer II (Zea mays) |
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Samples (4)
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Relations |
BioProject |
PRJNA167802 |
SRA |
SRP013491 |