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Series GSE38188 Query DataSets for GSE38188
Status Public on May 31, 2012
Title Comprehensive Analysis of Genes Expressed by Rare Microchimeric Fetal Cells in Maternal Mouse Lung
Organism Mus musculus
Experiment type Expression profiling by array
Summary During pregnancy, cells from each fetus travel into the maternal circulation and organs, resulting in the development of microchimerism. Identification of the cell types in this microchimeric population would permit better understanding of possible mechanisms by which they affect maternal health. However, comprehensive analysis of fetal cells has been hampered by their rarity. In this study, we sought to overcome this obstacle by combining flow cytometry with multidimensional gene expression microarray analysis of fetal cells isolated from the murine maternal lung during late pregnancy. Fetal cells were collected from the lungs of pregnant female mice. cDNA was amplified and hybridized to gene expression microarrays. The resulting fetal cell core transcriptome was interrogated using multiple methods including Ingenuity Pathway Analysis, the BioGPS gene expression database, principal component analysis, the Eurexpress gene expression atlas and primary literature. Here we report that small numbers of fetal cells can be flow sorted from the maternal lung, facilitating discovery-driven gene expression analysis. We additionally show that gene expression data can provide functional information about the fetal cells. Our results suggest that fetal cells in the murine maternal lung are a mixed population, consisting of trophoblasts, mesenchymal stem cells and cells of the immune system. The detection of trophoblasts and immune cells in the maternal lung may facilitate future mechanistic studies related to the development of immune tolerance and pregnancy-related complications, such as preeclampsia. Furthermore, the presence and persistence of mesenchymal stem cells in maternal organs may have implications for long-term postpartum maternal health.
Comprehensive gene expression microarray analysis of fetal cells isolated from the pregnant murine maternal lung.
 
Overall design Seven individual replicates were performed.
 
Contributor(s) Pritchard S, Wick HC, Slonim DK, Johnson KL, Bianchi DW
Citation(s) 22674387
Submission date May 23, 2012
Last update date Feb 11, 2019
Contact name Stephanie Pritchard
E-mail(s) stephanie.pritchard@tufts.edu
Phone 617-636-9120
Organization name Tufts University
Department Genetics
Lab Diana W. Bianchi Lab
Street address 800 Washington St, Box 396
City Boston
State/province MA
ZIP/Postal code 02111
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (7)
GSM936400 fetalcellsinlung_1
GSM936401 fetalcellsinlung_2
GSM936402 fetalcellsinlung_3
Relations
BioProject PRJNA167338

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE38188_RAW.tar 23.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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