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| Status |
Public on Sep 18, 2012 |
| Title |
Gene expression of the pigmented and non-pigmented epithelia of the ciliary body of human healthy eyes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The ciliary body (CB) of the human eye consists of the non-pigmented (NPE) and pigmented (PE) neuro-epithelia. We investigated the gene expression of the NPE and PE, to shed light on the molecular mechanisms underlying the most important functions of the CB. Therefore we isolated NPE and PE cells from seven healthy human donor eyes using laser dissection microscopy. Next, we performed RNA isolation, amplification, labeling and hybridization against 44k Agilent microarrays.
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| Overall design |
We performed the microarrays against a common reference sample, namely human RPE/choroid RNA. We performed 7 replicates of PE samples from 7 different donors and 7 NPE replicates from the same 7 different donors.
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| Contributor(s) |
Janssen SF, Bergen AA |
| Citation(s) |
23028713 |
| |
| Submission date |
May 14, 2012 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Sarah Janssen |
| E-mail(s) |
s.janssen@nin.knaw.nl
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| Organization name |
Netherlands Institute for Neuroscience
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| Department |
Ophthalmogenetics
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| Street address |
Meibergdreef 47
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| City |
Amsterdam |
| ZIP/Postal code |
1105 BA |
| Country |
Netherlands |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA166853 |
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