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| Status |
Public on Aug 28, 2012 |
| Title |
Sirt1, p53 and p38MAPK are crucial regulators of detrimental phenotypes of ESCs with Max expression ablation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Ablation of expression of the Max gene encoding a Myc protein partner in ES cells provoked two major phenomena, i.e. loss of pluripotency and apoptotic cell death. We found that nicotinamide (Nam) significantly alleviates these Max expression ablation-coupled phenotypes in ES cells. To see the alleviation effect of Nam on the overall expression profile of Max-null ES cells whose Max expression is controlled by the tet-off system, we eliminated Max expression by adding doxycycline (Dox) in the presence of Nam.
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| Overall design |
DNA microarray analyses were performed using total RNAs from Nam (4 mM)-treated Max-null ES cells that were cultured in the presence or absence of doxycycline for 6 days.
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| Contributor(s) |
Hishida T, Nakachi Y, Mizuno Y, Okazaki Y, Okuda A |
| Citation(s) |
22696478 |
| |
| Submission date |
May 10, 2012 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Akihiko Okuda |
| E-mail(s) |
akiokuda@saitama-med.ac.jp
|
| Phone |
81-42-984-4787
|
| Organization name |
Saitama Medical University
|
| Department |
Research Center for Genomic Medicine
|
| Lab |
Division of Biomedical Sciences
|
| Street address |
1397-1 Yamane
|
| City |
Hidaka |
| State/province |
Saitama |
| ZIP/Postal code |
3501241 |
| Country |
Japan |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA165155 |
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