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Series GSE37601 Query DataSets for GSE37601
Status Public on Jan 01, 2014
Title MBD-Seq of the V/SVZ and the CP at E16
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Summary Cell differentiation and proliferation are mutually exclusive. Although differentiating neurons are recognized as post-mitotic non-dividing cells, some Rb- and Rb family (Rb, p107, and p130)-deficient differentiating neurons proliferate and form tumor. Here, we found that the acute inactivation of all Rb family in differentiating cortical excitatory neurons caused radial migration defect and S-phase progression but not cell division, whereas that in cortical progenitors caused the cell division of the differentiating neurons generated from Rb –/–; p107 –/–; p130 –/– (Rb-TKO) progenitors. Genome-wide DNA methylation analysis revealed that proximal promoters tended to become methylated during differentiation in vivo. DNA demethylation by DNA methyltransferase inhibitor allowed the acutely inactivated Rb-TKO differentiating neurons to undergo G2/M-phase progression. Our finding illustrate that cortical excitatory neurons epigenetically lose their proliferative potency after neurogenesis.
Overall design 1 sample of the V/SVZ tissue and the CP tissue
Contributor(s) Ajioka I, Oshikawa M
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Submission date Apr 26, 2012
Last update date May 15, 2019
Contact name Itsuki Ajioka
Organization name Tokyo Medical and Dental University
Department Center for Brain Integration Research
Street address 1-5-45 Yushima, Bunkyo-ku
City Tokyo
ZIP/Postal code 113-8510
Country Japan
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (2)
GSM923103 01_VZ1
GSM923104 02_CP1
This SubSeries is part of SuperSeries:
GSE37625 V/SVZ (ventricular/subventricular zone) tissue and CP (cortical plate) tissue at E16
BioProject PRJNA162421
SRA SRP012554

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE37601_RAW.tar 14.2 Mb (http)(custom) TAR (of XLS)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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