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| Status |
Public on Apr 18, 2012 |
| Title |
Let-7b regulates skeletal muscle growth, development and fat deposition in deletion-type dwarf chickens (7-week-old chickens) |
| Organism |
Gallus gallus |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
A deletion mutation in the growth hormone receptor (GHR) gene results in the inhibition of skeletal muscle growth and fat deposition in dwarf chickens. In this study, microarray techniques were used to detect the miRNA and mRNA expression profiles of 14-day-old embryo and 7-week-old chicken skeletal muscle of deletion-type dwarf chickens and normal-type chickens. Skeletal muscle tissues of Dwarf recessive White Rock chickens and normal recessive White Rock chickens were used to make the microarray assay. Results show the expression of miR-1623 and miR-181b in 14-day-old embryos and of let-7b and miR-128 in 7-week-old chickens. let-7b was the only miRNA found to be completely complementary to its target in the 3'UTR of GHR and inhibited GHR gene expression. KEGG (Kyoto Encyclopaedia of Genes and Genomes) pathway analysis and RT-PCR verified that there were three main signalling pathways regulating the skeletal muscle growth and fat deposition of chickens influenced by the let-7b-regulated GHR gene. The suppression of the cytokine signalling 3 (SOCS3) gene was found to be involved in the signalling pathway of adipocytokines. We found that let-7b is the critical miRNA involved in the regulation of the GHR gene. SOCS3 plays a critical role in the network regulating skeletal muscle growth and fat deposition via let-7b-mediated GHR gene expression.
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| Overall design |
Two groups were analyzed in the array assay: one group consisted of normal recessive White Rock 7-week-old chicken leg muscle tissues, and the other group consisted of dwarf recessive White Rock 7-week-old chicken leg muscle tissues. The control samples were labeled as A1b, A2b, A3b, and the dwarf chicken samples were labeled as B1b, B2b, and B3b. 9 total chickens per breed, 3 chickens used per breed for each sample. 523 mature miRNA sequences were assembled and integrated into the LC miRNA microarray design, and different expression miRNAs were measured on the 7000HT Fast Real-Time PCR system.
REPLACE
This submission represents the miRNA profiling component of the study.
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| Contributor(s) |
Lin S, Li H, Zhang X |
| Citation(s) |
22781587 |
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| Submission date |
Apr 17, 2012 |
| Last update date |
Jan 22, 2014 |
| Contact name |
Hongmei Li |
| E-mail(s) |
lhm-lhm@sohu.com
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| Phone |
13316044567
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| Organization name |
south china agricultural university
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| Street address |
Guangzhou tianhe wushan street
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| City |
Guangzhou |
| ZIP/Postal code |
510640 |
| Country |
China |
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| Platforms (1) |
| GPL15455 |
LC Sciences Gallus gallus miRChicken_15 |
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| Samples (3) |
| GSM916831 |
7-week-old chicken leg muscle (normal recessive White Rock chicken / dwarf recessive White Rock chicken), rep1 |
| GSM916832 |
7-week-old chicken leg muscle (normal recessive White Rock chicken / dwarf recessive White Rock chicken), rep2 |
| GSM916833 |
7-week-old chicken leg muscle (normal recessive White Rock chicken / dwarf recessive White Rock chicken), rep3 |
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| This SubSeries is part of SuperSeries: |
| GSE37368 |
Let-7b regulates skeletal muscle growth, development and fat deposition in deletion-type dwarf chickens |
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| Relations |
| BioProject |
PRJNA159823 |
