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| Status |
Public on May 21, 2012 |
| Title |
A Computational Profiling of Changes in Gene Expression and Transcription Factors Induced by vFLIP K13 in Primary Effusion Lymphoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Infection of Kaposi's sarcoma associated herpes virus (KSHV) has been linked to the development of primary effusion lymphoma (PEL), which is characterized by the loss of expression of B cell markers and effusions in the body cavities. This unique clinical feature of PEL has been attributed to their distinctive gene expression profile which shows overexpression of genes in various signaling pathways. KSHV-encoded latent protein vFLIP K13 has been shown to promote the survival and proliferation of PEL cells. In this study, we have employed gene array analysis followed by bioinformatics analysis of coordinated transcriptional factors network as well as biological pathways to characterize the effect of K13 on PEL-derived BCBL1 cells. We observed that genes associated with Cytokine signaling, Cell death, NF-kappaB and Cell adhesion pathways were differentially regulated by K13.
We used computational approach complemented with microarrays to detail the global gene expression and identified distinct classes of differentially regulated genes in various cellular processes.
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| Overall design |
We employed gene array analysis followed by bioinformatics analyses of coordinated transcriptional factors network as well as biological pathways to characterize the effect of K13 on in PEL-derived BCBL1 cells.
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| |
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| Contributor(s) |
Punj V, Matta H, Chaudhary PM |
| Citation(s) |
22624040 |
| |
| Submission date |
Apr 17, 2012 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Vasu Punj |
| E-mail(s) |
vasupunj@gmail.com
|
| Organization name |
USC
|
| Street address |
Biggy Street
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90033 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (4)
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| GSM916571 |
BCBL1 cells stably expressing K13-ER with 4OHT-inducible ER system-untreated |
| GSM916572 |
BCBL1 cells stably expressing K13-ER with 4OHT-inducible ER system-treated with 4OHT |
| GSM916573 |
BCBL1 cells stably expressing empty vector-untreated |
| GSM916574 |
BCBL1 cells stably expressing empty vector-treated with 4OHT |
|
| Relations |
| BioProject |
PRJNA159727 |
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