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Series GSE37229 Query DataSets for GSE37229
Status Public on Sep 27, 2012
Title c-Myc is a universal amplifier of gene expression [ChIP-Seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The c-Myc HLH-bZIP protein has been implicated in physiological or pathological growth, proliferation, apoptosis, metabolism and differentiation at the cellular, tissue or organismal levels via regulation of numerous target genes. In part due to the incomplete inventory and functional accounting of Myc’s targets, no principle unifies Myc action. To relate the dynamics of Myc-binding with target expression and function in a system where Myc-levels are temporally and physiologically regulated, the transcriptomes and the genome-wide distributions of Myc, RNA polymerase II and chromatin modifications were compared during lymphocyte activation and in ES cells. A remarkably simple rule emerged from this quantitative analysis: Myc is not an on-off switch, but is a non-linear amplifier of expression, acting universally at active genes, except for immediate early genes that are strongly induced before Myc. This rule of Myc action explains the vast majority of Myc biology observed in literature.
Overall design Quiescent B-cells (B0) were treated with lipopolysaccharide (LPS) for 4 hrs (B4), and resting T-cells (T0) were activated with conA for 4 hrs (T4) and for 14 hrs (T14). Following treatments, RNA was harvested and chromatin was prepared at these time points. The chromatin was immunoprecipitated using anti-EGFP antibodies and analyzed by ChIP-seq.
Contributor(s) Nie Z, Hu G, Wei G, Cui K, Yamane A, Resch W, Wang R, Green DR, Tessarollo L, Casellas R, Zhao K, Levens D
Citation(s) 23021216
Submission date Apr 12, 2012
Last update date May 15, 2019
Contact name Gangqing Hu
Organization name West Virginia University
Department MicroBiology, Immunology, and Cell Biology
Lab 2072A, HSC North, Floor 2
Street address 64 Medical Center Drive
City Morgantown
State/province West Virginia
ZIP/Postal code 26506-9177
Country USA
Platforms (3)
GPL9185 Illumina Genome Analyzer (Mus musculus)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (16)
GSM914081 B4_Myc_ChIPSeq
GSM914082 B4_IgG_ChIPSeq
GSM914083 T4_Myc_ChIPSeq
This SubSeries is part of SuperSeries:
GSE37230 c-Myc is a universal amplifier of gene expression
SRA SRP012171
BioProject PRJNA159017

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Supplementary file Size Download File type/resource
GSE37229_RAW.tar 402.9 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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