|
| Status |
Public on Apr 25, 2012 |
| Title |
m6A mapping in human RNA (with treatments) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We developed a novel approach, m6A-seq, for high-resolution mapping of the transcriptome-wide m6A landscape, based on antibody-mediated capture followed by massively parallel sequencing.
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| Overall design |
Identification of m6A modified sequences in HepG2 cells. HepG2 cells were incubated with either IFNg (200ng/ml) or HGF/SF (10 ng/ml) over night. Stress effects were tested in HepG2 cells by either 30 minutes incubation at 43ºC (heat shock) or UV irradiation of 0.04 J/cm2 followed by 4 hours of recovery in normal growing conditions prior to harvesting using Trypsin.
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| |
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| Contributor(s) |
Dominissini D, Moshitch-Moshkovitz S, Schwartz S, Salmon-Divon M, Cesarkas K, Rechavi G |
| Citation(s) |
22575960 |
| |
| Submission date |
Apr 03, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Sharon Moshitch-Moshkovitz |
| E-mail(s) |
sharon.moshkovitz@sheba.health.gov.il
|
| Organization name |
Sheba Medical Center
|
| Department |
Cancer Research Center
|
| Street address |
Laboratory wing
|
| City |
Tel-Hashomer |
| ZIP/Postal code |
52621 |
| Country |
Israel |
| |
|
| Platforms (1) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
|
| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE37005 |
Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq |
|
| Relations |
| BioProject |
PRJNA158109 |
| SRA |
SRP012098 |
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