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Series GSE37002 Query DataSets for GSE37002
Status Public on Apr 25, 2012
Title m6A mapping in human RNA (with treatments)
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We developed a novel approach, m6A-seq, for high-resolution mapping of the transcriptome-wide m6A landscape, based on antibody-mediated capture followed by massively parallel sequencing.
Overall design Identification of m6A modified sequences in HepG2 cells. HepG2 cells were incubated with either IFNg (200ng/ml) or HGF/SF (10 ng/ml) over night. Stress effects were tested in HepG2 cells by either 30 minutes incubation at 43ÂșC (heat shock) or UV irradiation of 0.04 J/cm2 followed by 4 hours of recovery in normal growing conditions prior to harvesting using Trypsin.
Contributor(s) Dominissini D, Moshitch-Moshkovitz S, Schwartz S, Salmon-Divon M, Cesarkas K, Rechavi G
Citation(s) 22575960
Submission date Apr 03, 2012
Last update date May 15, 2019
Contact name Sharon Moshitch-Moshkovitz
Organization name Sheba Medical Center
Department Cancer Research Center
Street address Laboratory wing
City Tel-Hashomer
ZIP/Postal code 52621
Country Israel
Platforms (1)
GPL10999 Illumina Genome Analyzer IIx (Homo sapiens)
Samples (8)
GSM908329 HepG2_UV_IP
GSM908330 HepG2_UV_Input
GSM908331 HepG2_HS_IP
This SubSeries is part of SuperSeries:
GSE37005 Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq
BioProject PRJNA158109
SRA SRP012098

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Supplementary file Size Download File type/resource
GSE37002_Supplementary_data_HepG2_treatments.txt.gz 1.8 Mb (ftp)(http) TXT
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