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Series GSE36919 Query DataSets for GSE36919
Status Public on Jul 01, 2012
Title MCMV infection of cultured mouse cells induces expression of miR-7a.
Organism Mus musculus
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary One goal of viral infection is to reprogram the host cell to optimize viral replication. As part of this process, viral miRNAs may compete for components of the miRNA/siRNA pathway as well as regulate cellular targets. Mouse Cytomegalovirus has been described to generate large numbers of viral miRNAs during lytic infection and was therefore used to analyze the impact of viral miRNAs on the host cell small RNA system as well as to check for sorting of viral small RNAs into specific Ago-proteins. Deep sequencing analysis of MCMV infected cells revealed that viral miRNAs represent only app. 13% of all detected miRNAs. All previously described MCMV miRNAs with the exception of miR-m88-1* were confirmed and for the MCMV miR-m01-1 hairpin an additional miRNA, designated miR-m01-1-3p, was found. Its presence was confirmed by qPCR and Northern Blot. Deep sequencing after RISC IP with antibodies specific for either Ago1 or Ago2 showed that all MCMV miRNAs are loaded into both RISC complexes. The ratio of MCMV to mouse miRNAs was not increased after immunoprecipitation of Ago-proteins. Viral miRNAs therefore do not overwhelm the host miRNA processing system nor are they preferentially incorporated into RISC. We found that 3 mouse miRNAs showed an altered expression due to MCMV infection. Down-regulation of miR-27a, as previously described, could be confirmed. In addition, miR-26a was down-regulated and an up-regulation of miR-7a dependent on viral protein expression could be observed.
Overall design Examination of small RNA expression in uninfected vs. infected cells, immunoprecipitation + sequencing of Ago1 and Ago2 loaded small RNAs in infected cells
Contributor(s) Dittmer A, Förstemann K
Citation(s) 22442111
Submission date Mar 29, 2012
Last update date May 15, 2019
Contact name Klaus Förstemann
Organization name Ludwig-Maximilians University
Department Gene Center
Lab Förstemann
Street address Feodor-Lynen 25
City Munich
ZIP/Postal code 81377
Country Germany
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (4)
GSM906323 mock infected cells
GSM906324 MCMV infected cells
GSM906325 IP Ago1
BioProject PRJNA157095
SRA SRP011992

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