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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jan 11, 2006 |
| Title |
Gene profiling analysis of mouse splenic DC subpopulations |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To characterize differences between BALB/c splenic CD11cintB220+Gr1+ PDCs (plasmacytoid dendritic cells), CD11cintB220+CD49b+ IKDCs (interferon producing killer-dendritic cells), and CD11chighB220- cDCs (conventional dendritic cells), we performed gene expression profile analysis using Affymetrix chips. We FACS-sorted BALB/c spleen DC subpopulations. Comparison of differentially expressed genes between IKDCs and cDCs vividly revealed selective expression of multiple NK-related genes in IKDCs . These included granzymes A, B, K and M, perforin, Fas ligand, and NK receptors such as NKG2A, NKG2D, Ly49 family genes, NKR-P1, NKG7, NKp46 and Mafa (KLRG1). No NK-related genes were highly expressed in the PDCs.
Keywords: Cell type comparison
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| Overall design |
We prepared two biological samples separately for each DC population, and analyzed the expression profiles by comparing to those of cDCs (control sample).
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| Contributor(s) |
Chan CW, Crafton E, Pardoll D, Housseau F |
| Citation(s) |
16444266 |
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| Submission date |
Nov 28, 2005 |
| Last update date |
Feb 18, 2018 |
| Contact name |
Drew Pardoll |
| E-mail(s) |
dmpardol@jhmi.edu
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| Phone |
410 955-1500
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| Fax |
410 955-1500
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| Organization name |
Drew Pardoll
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| Street address |
orleans st
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| City |
baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
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| Platforms (3) |
| GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
| GPL82 |
[MG_U74Bv2] Affymetrix Murine Genome U74B Version 2 Array |
| GPL83 |
[MG_U74Cv2] Affymetrix Murine Genome U74 Version 2 Array |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA93837 |
| Supplementary data files not provided |
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