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| Status |
Public on Jun 17, 2013 |
| Title |
Effect of human breast tumor supernatant on normal CD4+ T cells (Confirmation Set SN) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
CD4+ helper T (Th) cells are critical regulators of immune responses but their role in breast cancer is currently unknown. This work aims to characterize Th cells infiltrating invasive primary human breast tumors, analyze the influence by the tumor microenvironment and identify Th cell specific prognostic gene signatures. CD4+ T cells isolated from the tumor (TIL), axillary lymph node (LN) and blood (PB) of 10 patients were analyzed on Affymetrix U133 Plus 2.0 arrays. A confirmation set of 60 patients were studied by flow cytometry, qRT-PCR or immunohistochemistry and analyzed according to the extent of the tumor immune infiltrate. Gene expression profiles of freshly isolated TIL were also compared with TIL that had been rested overnight or with CD4+ T cells [non-stimulated (NS) or stimulated (S)] from healthy donor PB treated with tumor supernatant (SN).
Comparing gene expression profiles of donor blood derived memory CD4+ T cells [non-stimulated (NS) or stimulated (S)] with and without tumor supernatant (SN) treatment
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| Overall design |
Memory CD4+ T cells isolated from a healthy donor blood (NS or S) were treated (and as control: untreated samples in biological triplicate) with SN obtained from fresh breast tumor homogenates of 4 patients and analyzed on Affymetrix U133 Plus 2.0 arrays
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| |
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| Contributor(s) |
Willard-Gallo K |
| Citation(s) |
23778140 |
| |
| Submission date |
Mar 23, 2012 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Chunyan GU |
| E-mail(s) |
chunyan.gu@bordet.be
|
| Phone |
+32(0)25413711
|
| Organization name |
Free University of Brussels
|
| Department |
Medecine (Jules Bordet Institute)
|
| Lab |
Molecular Immunology
|
| Street address |
Bld de Waterloo 127
|
| City |
Brussels |
| ZIP/Postal code |
1000 |
| Country |
Belgium |
| |
|
| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (14)
|
| GSM900560 |
Memory CD4 NS at 24h, biological rep1 |
| GSM900561 |
Memory CD4 NS at 24h, biological rep2 |
| GSM900562 |
Memory CD4 NS at 24h, biological rep3 |
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| This SubSeries is part of SuperSeries: |
| GSE36769 |
CD4+ TIL in human breast cancer |
|
| Relations |
| BioProject |
PRJNA156785 |
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