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| Status |
Public on Mar 13, 2012 |
| Title |
Expression data from germinating conidia of Aspergillus niger in the presence of the polyene antibiotic natamycin. |
| Platform organism |
Aspergillus niger CBS 513.88 |
| Sample organism |
Aspergillus niger |
| Experiment type |
Expression profiling by array
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| Summary |
The impact of natamycin on Aspergillus niger was analysed during 8 h of germination of conidia. Polarization, germ tube formation, and mitosis were inhibited in the presence of 3 µM and 10 µM of the anti fungal compound natamycin, while at 10 µM also isotropic growth was affected. Natamycin did not have an effect on the decrease of internal microviscosity and the concomitant reduction on mannitol and trehalose levels. However, it did abolish the increase of intracellular levels of glycerol and glucose during the 8 h period of germination. Natamycin hardly affected the changes that occur in the RNA profile during the first 2 h of germination. During this time period, genes related to transcription, protein synthesis, energy and cell cycle and DNA processing were particularly up regulated. On the other hand, when 8 h old germlings were compared, genes involved in ergosterol biosynthesis were down regulated while genes related to endocytosis and metabolism of compatible solutes, and genes encoding protective proteins were up regulated.
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| Overall design |
Samples of germinating conidia of Aspergillus niger N402 were taken in triplo at 0h (dormant), 2h, and 8h after inoculation in CM. These were compared with conidia that germinated in the presence of either 3 µM or 10 µM natamycin.
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| Contributor(s) |
van Leeuwen MR, Krijgsheld P, Wyatt TT, Golovina EA, Menke H, Dekker A, Stark J, Stam H, Bleichrodt RJ, Wösten HA, Dijksterhuis J |
| Citation(s) |
23449730 |
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M. R. van Leeuwen, P. Krijgsheld, T. T. Wyatt, E. A. Golovina, H. Menke, A. Dekker, J. Stark, H. Stam, R. Bleichrodt, H. A. B. W sten and J. Dijksterhuis. The effect of natamycin on the transcriptome of conidia of Aspergillus niger. (2012). Studies in Mycology 74: 71-85; doi:10.3114/sim0013.
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| Submission date |
Mar 12, 2012 |
| Last update date |
Feb 12, 2020 |
| Contact name |
Martin Richard van Leeuwen |
| E-mail(s) |
r.vanleeuwen@cbs.knaw.nl
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| Phone |
+31 (0)30 2122600
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| Fax |
+31 (0)30 2512097
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| Organization name |
CBS-KNAW/Fungal Biodiversity Centre
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| Department |
Applied and Industrial Mycology
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| Street address |
Uppsalalaan 8
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| City |
Utrecht |
| ZIP/Postal code |
3584 CT |
| Country |
Netherlands |
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| Platforms (1) |
| GPL6758 |
[dsmM_ANIGERa_anColl] DSM Aspergillus niger CBS513.88 14k v1 |
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| Samples (21)
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| GSM893521 |
conidia at T2, biological rep1 |
| GSM893522 |
conidia at T2, biological rep2 |
| GSM893523 |
conidia at T2, biological rep3 |
| GSM893530 |
conidia at T8, biological rep1 |
| GSM893531 |
conidia at T8, biological rep2 |
| GSM893532 |
conidia at T8, biological rep3 |
| GSM893533 |
conidia at T2, in the presence of 3 µM natamycin, biological rep1 |
| GSM893534 |
conidia at T2, in the presence of 3 µM natamycin, biological rep2 |
| GSM893535 |
conidia at T2, in the presence of 3 µM natamycin, biological rep3 |
| GSM893536 |
conidia at T8, in the presence of 3 µM natamycin, biological rep1 |
| GSM893537 |
conidia at T8, in the presence of 3 µM natamycin, biological rep2 |
| GSM893538 |
conidia at T8, in the presence of 3 µM natamycin, biological rep3 |
| GSM893539 |
conidia at T2, in the presence of 10 µM natamycin, biological rep1 |
| GSM893540 |
conidia at T2, in the presence of 10 µM natamycin, biological rep2 |
| GSM893541 |
conidia at T2, in the presence of 10 µM natamycin, biological rep3 |
| GSM893542 |
conidia at T8, in the presence of 10 µM natamycin, biological rep1 |
| GSM893543 |
conidia at T8, in the presence of 10 µM natamycin, biological rep2 |
| GSM893544 |
conidia at T8, in the presence of 10 µM natamycin, biological rep3 |
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| Relations |
| BioProject |
PRJNA153183 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE36440_RAW.tar |
45.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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