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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jun 12, 2012 |
| Title |
Global changes in gene expression in dermal fibroblasts with in vivo and in vitro deletion of the RBP-Jk gene |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
It is currently unclear whether tissue changes surrounding multifocal epithelial tumors are a cause or consequence of cancer. Here, we provide evidence that loss of mesenchymal Notch/CSL signaling causes tissue alterations, including stromal atrophy and inflammation, which precede and are potent triggers for epithelial tumors. Mice carrying a mesenchymal-specific deletion of CSL/RBP-JK, a key Notch effector, exhibit spontaneous multifocal keratinocyte tumors that develop after dermal atrophy and inflammation. CSL-deficient dermal fibroblasts promote increased tumor cell proliferation through up-regulation of c-Jun and c-Fos expression and consequently higher levels of diffusible growth factors, inflammatory cytokines, and matrix remodeling enzymes. In human skin samples, stromal fields adjacent to cutaneous squamous cell carcinomas and multifocal premalignant actinic keratosis lesions exhibit decreased Notch/CSL signaling and associated molecular changes. Importantly, these changes in gene expression are also induced by UVA, a known environmental cause of cutaneous field cancerization and skin cancer.
We used microarrays to detail the global changes in gene expression in dermal fibroblasts with in vivo and in vitro deletion of the RBP-Jk gene, compared to corresponding controls
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| Overall design |
Global changes in gene expression in dermal fibroblasts with in vivo and in vitro deletion of the RBP-Jk gene were assessed, in parallel with the corresponding controls
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| Contributor(s) |
Hu B, Castillo E, Harewood L, Ostano P, Reymond A, Dummer R, Raffoul W, Hoetzenecker W, Hofbauer GF, Dotto GP |
| Citation(s) |
22682244 |
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| Submission date |
Mar 08, 2012 |
| Last update date |
Feb 11, 2019 |
| Contact name |
giovanna chiorino |
| E-mail(s) |
giovanna.chiorino@gmail.com
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| Organization name |
Fondo Edo Tempia
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| Department |
Cancer Genomics
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| Street address |
via malta 3
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| City |
Biella |
| ZIP/Postal code |
13900 |
| Country |
Italy |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (4)
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| GSM889563 |
mouse fibroblasts Adeno Cre in vitro |
| GSM889564 |
mouse fibroblasts Adeno GFP in vitro |
| GSM889565 |
mouse fibroblasts ColI Cre x RBP loxp-loxp in vivo |
| GSM889566 |
mouse fibroblasts RBP loxp-loxp in vivo |
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| Relations |
| BioProject |
PRJNA153325 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE36359_RAW.tar |
14.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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