GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE36241 Query DataSets for GSE36241
Status Public on Sep 19, 2012
Title Identification of a FOXO3/IRF7 circuit that limits inflammatory sequelae of antiviral responses (ChIP-Seq)
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We predict that a member of the forkhead family of transcription factors, FOXO3, is a negative regulator of a subset of antiviral genes. This prediction was validated using macrophages isolated from Foxo3-null mice. Genome-wide location analysis combined with gene deletion studies identified the Irf7 gene as a critical target of FOXO3. FOXO3 was identified as a negative regulator of Irf7 transcription. Our data suggest that the FOXO3-IRF7 regulatory circuit represents a novel mechanism for establishing the requisite set points in the interferon pathway.
Overall design C57BL/6 mice were obtained from Jackson Laboratories. Foxo3-/- mice in the FVB background were obtained from MMRRC and were backcrossed to C57BL/6 mice at least 5 times to generate congenic mice. C57BL/6 Foxo3+/- heterozygotes were intercrossed to generate Foxo3-/- mice. Mice were maintained at the animal facility of the Institute for Systems Biology and used at 8–12 weeks of age. All animals were housed and handled according to the approved protocols of University of Washington and Institute for Systems Biology's Institutional Animal Care and Use Committees.
Contributor(s) Raman A, Litvak V
Citation(s) 22982991
Submission date Mar 02, 2012
Last update date May 15, 2019
Contact name Ayush Raman
Phone 206-732-1473
Organization name Institute for Systems Biology
Department Computational Dept.
Lab Shmulevich Lab
Street address 401 Terry Avenue North
City Seattle
State/province WA
ZIP/Postal code 98105
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (3)
GSM884978 Unstimulated macrophages Foxo3a Wildtype ChIP-Seq
GSM884979 Unstimulated macrophages H4Ac Wildtype ChIP-Seq
GSM884980 Unstimulated macrophages H4Ac Foxo3 Knockout ChIP-Seq
This SubSeries is part of SuperSeries:
GSE37052 Identification of a FOXO3/IRF7 circuit that limits inflammatory sequelae of antiviral responses
SRA SRP011232
BioProject PRJNA153161

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE36241_RAW.tar 2.4 Gb (http)(custom) TAR (of BAR, BED, TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap