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Series GSE36033 Query DataSets for GSE36033
Status Public on Feb 24, 2012
Title The ATP-P2X7 signaling axis is dispensable for obesity-associated inflammasome activation in adipose tissue
Organism Mus musculus
Experiment type Expression profiling by array
Summary Inflammasome activation in adipose tissue has been implicated in obesity-associated insulin resistance and type 2 diabetes. However, when and how inflammasome is activated in adipose tissue remains speculative. Here we test the hypothesis that extracellular ATP, a potent stimulus of inflammasome in macrophages via purinergic receptor P2X, ligand-gated ion channel, 7 (P2X7), may play a role in inflammasome activation in adipose tissue in obesity. Our data show that inflammasome is activated in adipose tissue upon 8-week feeding of 60% HFD, coinciding with the onset of hyperglycemia and hyperinsulinemia as well as the induction of P2X7 in adipose tissue. Unexpectedly, P2X7-deficient animals on HFD exhibit no changes in metabolic phenotypes, nor in inflammatory responses or inflammasome activation when compared to the wildtype controls. Similar observations have been obtained in hematopoietic cell-specific P2X7-deficient animals generated by bone marrow transplantation. Thus, we conclude that inflammasome activation in adipose tissue in obesity coincides with the onset of hyperglycemia and hyperinsulinemia, but unexpectedly, is not mediated by the ATP-P2X7 signaling axis. The nature of the inflammasome-activating danger signal(s) in adipose tissue in obesity remains to be characterized.
Overall design Wild type and P2X7 knockout mice were fed a low fat diet (chow) or high fat diet for 12 weeks. After the diet intervention period, the animals were killed and epididymal white adipose tissue was removed. Total RNA was isolated and subjected to gene expression profiling.
Contributor(s) Sun S, Xia S, Ji Y, Kersten S, Qi L
Citation(s) 22415881
Submission date Feb 23, 2012
Last update date Apr 18, 2017
Contact name Guido Hooiveld
Organization name Wageningen University
Department Div. Human Nutrition & Health
Lab Nutrition, Metabolism & Genomics Group
Street address HELIX, Stippeneng 4
City Wageningen
ZIP/Postal code NL-6708WE
Country Netherlands
Platforms (1)
GPL11533 [MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version]
Samples (13)
GSM879477 WAT_WT_LFD_repl1
GSM879478 WAT_WT_LFD_repl2
GSM879479 WAT_WT_LFD_repl3
BioProject PRJNA151751

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Supplementary file Size Download File type/resource
GSE36033_RAW.tar 59.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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