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| Status |
Public on Mar 01, 2006 |
| Title |
Microarray analysis of in vivo inhibition of mir-122 with an antisense oligonucleotide. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Current understanding of microRNA (miRNA) biology is limited, and antisense oligonucleotide (ASO) inhibition of miRs is a powerful technique for their functionalization. To uncover the role of the liver-specific miR-122 in the adult liver, we inhibited it in vivo using a 2’-O-methoxyethyl phosphorothioate ASO. This microrarray experiment was used to identify changes in mRNA levels due to the inhibition of miR-122 that accompanied phenotypic observations of reduced cholesterol and triglycerides, increased fatty acid oxidation, and decreased fatty acid and cholesterol synthesis rates observed in hepatocytes.
Keywords: single treatment vs. control
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| Overall design |
The experiment contains two groups of 5 mice: one group treated with an antisense to miR-122, and a second group injected only with saline as a control. RNA was extracted from individual livers, and each of these samples was hybridized to a single-channel chip. The design was intended to permit determination of statistically significant fold changes 1.5 and above.
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| |
|
| Contributor(s) |
Esau C |
| Citation(s) |
16459310 |
| |
| Submission date |
Nov 11, 2005 |
| Last update date |
Mar 25, 2013 |
| Contact name |
Michael Pear |
| E-mail(s) |
mpear@isisph.com
|
| Phone |
760-603-2779
|
| URL |
http://www.isspharm.com
|
| Organization name |
Isis Pharmaceuticals, Inc.
|
| Department |
Research Informatics
|
| Street address |
1896 Rutherford Rd
|
| City |
Carlsbad |
| State/province |
CA |
| ZIP/Postal code |
92009 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL2897 |
GE Healthcare/Amersham Biosciences CodeLink™ Mouse Whole Genome Bioarray |
|
| Samples (10)
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| Relations |
| BioProject |
PRJNA93705 |
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