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Status |
Public on May 17, 2012 |
Title |
Age-dependent gene expression in isolated small intestinal epithelial cells (IECs) |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
Intestinal epithelial cells were isolated from total small intestine of each four 3- and 21-day-old C57BL/6 mice (protocol according to: Lotz et al., J. Exp. Med. 2006). Each two 3- and 21-day-old animals were obtained from one litter. Total RNA was isolated by TriZol and ist purity was examined using a Bioanalyszer. We used microarrays to detail the global gene expression in primary total isolated intestinal epithelial cells of 3- versus 21-day-old mice.
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Overall design |
Four biological replicates from isolated intestinal epithelial cells obtained from 3- and 21-day-old mice (each two animals from one litter). Colour change.
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Contributor(s) |
Hornef MW |
Citation(s) |
22570612, 25210785, 30089902 |
Submission date |
Feb 07, 2012 |
Last update date |
Nov 15, 2018 |
Contact name |
Mathias W. Hornef |
E-mail(s) |
hornef.mathias@mh-hannover.de
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Phone |
0049 511 538 9094
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Fax |
0049 511 532 4366
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Organization name |
Hannover Medical School
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Department |
Inst. of Medical Microbiology
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Street address |
Carl Neuberg Str. 1
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City |
Hannover |
ZIP/Postal code |
30625 |
Country |
Germany |
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Platforms (1) |
GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
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Samples (4)
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GSM871387 |
IEC day 3 (Cy3) versus IEC day 21 (Cy5) from litter A |
GSM871388 |
IEC day 3 (Cy5) versus IEC day 21 (Cy3) from litter A |
GSM871389 |
IEC day 3 (Cy3) versus IEC day 21 (Cy5) from litter B |
GSM871390 |
IEC day 3 (Cy5) versus IEC day 21 (Cy3) from litter B |
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Relations |
BioProject |
PRJNA152513 |