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Series GSE35496 Query DataSets for GSE35496
Status Public on Feb 20, 2013
Title ChIP-Seq of Sox2 and Brn2 in ESCs, NPCs, and differentiating ESCs
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We analyzed the genome-wide binding of Sox2 and POU factor partner factors, Oct4 in ESCs (using published datasets PMID:18692474 and GSM307137, GSM307154, GSM307155) and Brn2 in NPCs. We found that Sox2 and Oct4 co-occupied a large subset of promoters and enhancers in ESCs, but that Sox2 and Brn2 co-occupy predominantly enhancers. Further, we overexpressed Brn2 in differentiating ESCs and showed that ectopic Brn2 recruited Sox2 to NPC-specific targets, resulting in skewed differentiation towards the neural lineage.
Overall design Examination of transcription factor binding in ESCs, NPCs, and differentiating ESCs by ChIP-Seq.
Contributor(s) Lodato MA, Jaenisch R, Boyer LA
Citation(s) 23437007
Submission date Feb 02, 2012
Last update date May 15, 2019
Contact name Michael Lodato
Organization name Whitehead Institute
Street address 9 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
Platforms (2)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (30)
GSM1050286 Brn2 NPC ChIP-Seq, rep1
GSM1050287 Brn2 NPC ChIP-Seq, rep2
GSM1050288 Sox2 NPC ChIP-Seq, rep1
Reanalysis of GSM307154
Reanalysis of GSM307155
Reanalysis of GSM307137
SRA SRP010714
BioProject PRJNA152295

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE35496_Brn2_NPC_Wig_min0.1.WIG.gz 21.0 Mb (ftp)(http) WIG
GSE35496_Brn2_TetOBrn2_Day2_Wig_min0.1.WIG.gz 25.6 Mb (ftp)(http) WIG
GSE35496_Brn2_rtta_Day2_Wig_min0.1.WIG.gz 23.9 Mb (ftp)(http) WIG
GSE35496_H3K27Ac_TetOBrn2_Day2_Wig_min0.1.WIG.gz 19.1 Mb (ftp)(http) WIG
GSE35496_H3K27Ac_rtta_Day2_Wig_min0.1.WIG.gz 17.5 Mb (ftp)(http) WIG
GSE35496_H3K4me1_TetOBrn2_Day2_Wig_min0.1.WIG.gz 19.8 Mb (ftp)(http) WIG
GSE35496_H3K4me1_rtta_Day2_Wig_min0.1.WIG.gz 20.2 Mb (ftp)(http) WIG
GSE35496_Sox2_ESC_Wig_min0.1.WIG.gz 17.1 Mb (ftp)(http) WIG
GSE35496_Sox2_NPC_Wig_min0.1.WIG.gz 21.3 Mb (ftp)(http) WIG
GSE35496_Sox2_TetOBrn2_Day2_Wig_min0.1.WIG.gz 25.4 Mb (ftp)(http) WIG
GSE35496_Sox2_rtta_Day2_Wig_min0.1.WIG.gz 24.4 Mb (ftp)(http) WIG
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