|
| Status |
Public on Feb 03, 2012 |
| Title |
Gene expression profiling of Myf5-Cre;Smad4flox/flox mouse models of tongue development |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We investigated Smad4-mediated TGF-beta signaling in the development of occipital somite-derived myogenic progenitors during tongue morphogenesis by comparing the transcriptomes of tongue derived from Myf5-Cre;Smad4flox/flox mutant and Myf5-Cre;Smad4flox/+ control mice at day E13.5. Based on gene expression profiles and functional studies, we elucidated the influences Smad4 activity and TGF-beta signaling have on the gene expression profiles underlying tongue development. The data are consistent with the hypothesis that TGF-beta-Smad4-FGF6 signaling cascade plays a crucial role in myogenic cell fate determination and lineage progression during tongue myogenesis.
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| |
|
| Overall design |
We obtained RNA samples from tongue tissues of mutant and control mouse embryos (C57BL/6J) at day E13.5 RNA and subjected them to analysis on Affymetrix GeneChip Mouse Genome 430 2.0 Arrays.
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| Contributor(s) |
Han D, Chai Y, Hacia JG |
| Citation(s) |
22438570 |
| |
| Submission date |
Jan 26, 2012 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Joseph Gerard Hacia |
| E-mail(s) |
hacia@hsc.usc.edu
|
| Phone |
323-442-3030
|
| Organization name |
University of Southern California
|
| Department |
Biochemistry and Molecular Biology
|
| Lab |
Hacia Lab
|
| Street address |
2250 Alcazar Street, IGM 261
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90089 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (12)
|
| GSM866738 |
control tongue at E13.5, biological rep1 |
| GSM866739 |
control tongue at E13.5, biological rep2 |
| GSM866740 |
control tongue at E13.5, biological rep3 |
| GSM866741 |
control tongue at E13.5, biological rep4 |
| GSM866742 |
control tongue at E13.5, biological rep5 |
| GSM866743 |
control tongue at E13.5, biological rep6 |
| GSM866744 |
mutant tongue at E13.5, biological rep1 |
| GSM866745 |
mutant tongue at E13.5, biological rep2 |
| GSM866746 |
mutant tongue at E13.5, biological rep3 |
| GSM866747 |
mutant tongue at E13.5, biological rep4 |
| GSM866748 |
mutant tongue at E13.5, biological rep5 |
| GSM866749 |
mutant tongue at E13.5, biological rep6 |
|
| Relations |
| BioProject |
PRJNA152741 |
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