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| Status |
Public on Mar 14, 2012 |
| Title |
Gene expression in mice with conditional over-activation of hedgehog signaling in the ovary |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The objective of the study was to determine the cause of anovulation in transgenic mice with conditional over-activation of the hedgehog signaling pathway in the ovary. In Amhr2(cre/+)-SmoM2 transgenic mice, a constitutively active version of the hedgehog signal transducer, smoothened (SMO), known as SMOM2, is expressed in the ovary following cre-mediated recombination. The hypothesis tested is that changes in the neonatal ovary caused by over-activation of hedgehog signaling leads to the life-long anovulation phenotype in transgenic mice.
Microarrays were used to examine differences in gene expression in ovaries of two day old wild-type mice (Amhr2(+/+)-SmoM2) and transgenic mice (Amhr2(cre/+)-SmoM2) in which the hedgehog signaling pathway was conditionally over-activated in the ovary.
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| Overall design |
Two microarrays were performed. RNA samples were prepared from ovaries of 8 Amhr2(+/+)-SmoM2 control mice and 8 Amhr2(cre/+)-SmoM2 mutant mice on day two of age. RNA quality was assessed by measurement of ribosomal RNA on an Agilent 2100 Bioanalyzer.
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| Contributor(s) |
Quirk SM, Cowan RG, Ren Y |
| Citation(s) |
22402963 |
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| Submission date |
Jan 16, 2012 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Susan Quirk |
| E-mail(s) |
smq1@cornell.edu
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| Phone |
607-254-4607
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| Fax |
607-255-9829
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| Organization name |
Cornell University
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| Department |
Animal Science
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| Street address |
431 Morrison Hall
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| City |
Ithaca |
| State/province |
NY |
| ZIP/Postal code |
14853 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA150717 |
