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| Status |
Public on Jan 04, 2012 |
| Title |
Weighted gene co-expression network analysis on microarray data from subregions of zebra finch (Taeniopygia guttata) basal ganglia |
| Organism |
Taeniopygia guttata |
| Experiment type |
Expression profiling by array
|
| Summary |
Similarities between speech and birdsong make songbirds advantageous for investigating the neurogenetics of learned vocal communication; a complex phenotype likely supported by ensembles of interacting genes in cortico-basal ganglia pathways of both species. To date, only FoxP2 has been identified as critical to both speech and birdsong. We performed weighted gene co-expression network analysis on microarray data from singing zebra finches to discover gene ensembles regulated during vocal behavior. We found ~2,000 singing- regulated genes comprising 3 co-expression groups unique to area X, the basal ganglia subregion dedicated to learned vocal-motor behavior. These contained known targets of human FOXP2 and potential avian targets. We validated novel biological pathways for vocalization. Our findings show that higher-order gene co-expression patterns, rather than expression levels, molecularly distinguish area X from the ventral striato-pallidum during singing. The previously unknown structure of singing-driven networks enables prioritization of molecular interactors that likely bear on human motor disorders, especially those affecting speech.
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| Overall design |
Gene expression was measured in 2 basal ganglia sub-regions (area X & ventral striato-pallidum (VSP)) of 27 adult male zebra finches that sang different amounts of song over a 2hr period in the morning. 18 birds were allowed to sing freely, 9 birds were discouraged from singing by the presence of an investigator and those that sang fewer than 10 song motifs were considered “non-singers”.
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| Contributor(s) |
Hilliard AT, Miller JE, Fraley E, Horvath S, White SA |
| Citation(s) |
22325205, 23144607 |
| |
| Submission date |
Jan 03, 2012 |
| Last update date |
Sep 19, 2019 |
| Contact name |
Austin T Hilliard |
| E-mail(s) |
ahilliard@ucla.edu
|
| Organization name |
UCLA
|
| Street address |
610 Charles E Young Dr East
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
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| Platforms (1) |
| GPL15081 |
Agilent-019785 Custom Zebra Finch Microarray |
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| Samples (54)
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| Relations |
| BioProject |
PRJNA150249 |
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