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| Status |
Public on Aug 02, 2012 |
| Title |
Difference expression analyses before and after human fucosyltransferase gene 1 transfection into ovarian cancer clear cell line |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
75% ovarian epithelial tumor overexpresses Lewis y antigen, which notably strenthen such malignant biological behaviors as metastasis, drug resistance and proliferation. The key enzyme of Lewis y synthesis is human fucosyltransferase gene 1(hfut1). Through transfecting hfut1 into Lewis y low expressing cell line RMG-I, we got a pair of cell lines. We hypothesized that Lewis y antigen plays a major role in ovarian canceration . Using gene arrays, we comprehensively profiled mRNA differential expression between fut1-pretransfected and posttransfected clear cell of human epithelial OvCa. We expected that over-expression of lewis y antigen would lead to higher expression of genes associated with growth, metastasis,proliferation and so on.
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| Overall design |
we established ovarian tumor cell line RMG-I-H by stably transfecting human fucosyltransferase gene 1 into cell line RMG-I. RMG-I-H overexpresses Lewis y antigen, which is known as a cancer-related tetrasaccharide antigen. We profiled for gene expression both cells pretransfected and posttransfected.
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| Contributor(s) |
Feng Z, Liu J, Bei L |
| Citation missing |
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| |
| Submission date |
Dec 12, 2011 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Ziwei Feng |
| Organization name |
China Medical University
|
| Street address |
Sanhao street
|
| City |
Shenyang |
| ZIP/Postal code |
110004 |
| Country |
China |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (2) |
| GSM848243 |
Ovarian cancer clear cell line RMG-I-H |
| GSM848244 |
Ovarian cancer clear cell line RMG-I |
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| Relations |
| BioProject |
PRJNA149577 |
