|
| Status |
Public on Oct 12, 2005 |
| Title |
Murine ES Cells: Control vs. Triple-Fusion Transfected |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Transcriptional profiling of mouse embryonic stem cells comparing control untreated ES cells with ES cells transfected with a pUb-fluc-mrfp-ttk triple fusion reporter gene. The latter makes ES visualization possible by FACS and single cell microscopy, high throughput bioluminescence imaging, and PET. Goal was to determine the effects of triple-fusion (TF) gene transfection on global ES gene expression.
Keywords: Genetic modification
|
| |
|
| Overall design |
Two-condition experiment, ES vs. TF-ES cells. Biological replicates: 4 control, 3 transfected, independently grown and harvested. One replicate per array.
|
| |
|
| Contributor(s) |
Wu JC, Spin JM, Cao F, Lin S, Gheysens O, Chen IY, Tsalenko A, Ghambhir SS, Quertermous T |
| Citation(s) |
16390873 |
| |
| Submission date |
Oct 10, 2005 |
| Last update date |
Jan 17, 2013 |
| Contact name |
Joshua M. Spin |
| E-mail(s) |
jspin1@stanford.edu
|
| Phone |
650-498-6353
|
| Organization name |
Stanford University
|
| Department |
Internal Medicine/Cardiovascular Division
|
| Lab |
Philip S. Tsao Lab
|
| Street address |
300 Pasteur Drive
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1307 |
Agilent Mouse Development Oligo Microarray (G4120A) Reannotated 020504 |
|
| Samples (7)
|
| GSM77334 |
Control Embyronic Stem Cell Replicate 1 |
| GSM77335 |
Control Embyronic Stem Cell Replicate 2 |
| GSM77336 |
Control Embyronic Stem Cell Replicate 3 |
| GSM77337 |
Control Embyronic Stem Cell Replicate 4 |
| GSM77338 |
Triple-Fusion Transfected Embryonic Stem Cells Replicate 1 |
| GSM77339 |
Triple-Fusion Transfected Embryonic Stem Cells Replicate 2 |
| GSM77340 |
Triple-Fusion Transfected Embryonic Stem Cells Replicate 3 |
|
| Relations |
| BioProject |
PRJNA93465 |
Less...
More...