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Series GSE34274 Query DataSets for GSE34274
Status Public on Dec 09, 2011
Title Gene expression profile of right ventricles from adult wild type and Ezh2-deficient hearts
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Adult-onset diseases can be associated with in utero events, but mechanisms for this remain unknown. The polycomb histone methyltransferase, Ezh2, stabilizes transcription by depositing repressive marks during development that persist into adulthood, but its function in postnatal organ homeostasis is unknown. We show that Ezh2 stabilizes cardiac gene expression and prevents cardiac pathology by repressing the homeodomain transcription factor Six1, which functions in cardiac progenitors but is stably silenced upon cardiac differentiation. Ezh2 deletion in cardiac progenitors caused postnatal myocardial pathology and destabilized cardiac gene expression with activation of Six1-dependent skeletal muscle genes. Six1 induced cardiomyocyte hypertrophy and skeletal muscle gene expression. Furthermore, genetically reducing Six1 levels rescued the pathology of Ezh2-deficient hearts. Thus, Ezh2-mediated repression of Six1 in differentiating cardiac progenitors is essential for stable postnatal heart gene expression and homeostasis. Our results suggest that epigenetic dysregulation in embryonic progenitor cells predisposes to adult disease and dysregulated stress responses.
Overall design Four samples were analyzed. RNA was obtained from ventricles from two wild type and two Ezh2-deficient hearts.
Contributor(s) Bruneau BG, Delgado-Olguin P, Christodoulou D, Seidman CE, Seidman JG
Citation(s) 22267199
Submission date Dec 08, 2011
Last update date May 15, 2019
Contact name Gladstone Bioinformatics
Organization name Gladstone Institutes
Department Data Science and Biotechnology
Lab Bioinformatics Core
Street address 1650 Owens St
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (4)
GSM846186 RV_Mu1 (Mutant sample 1)
GSM846187 RV_Mu3 (Mutant sample 3)
GSM846188 RV_Wt1 (Wildtype sample 1)
SRA SRP009662
BioProject PRJNA149953

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Supplementary file Size Download File type/resource
GSE34274_Reads_Table_1_Original_RV_Mu1_RV_Mu3_RV_Wt1_RV_Wt2.expr.txt.gz 2.4 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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