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Series GSE34238 Query DataSets for GSE34238
Status Public on Dec 08, 2011
Title C2C12 murine myoblast cell line and 48 h 10 uM BIO treated C2C12 cellulate
Organism Mus musculus
Experiment type Expression profiling by array
Summary In urodele amphibians, limb regeneration involves the dedifferentiation of muscle myotubes into single cells that may acquire pluripotent potential. We have employed small molecules (myoseverin and BIO) to attempt to reproduce this behavior in mammalian muscle culture. C2C12 myotubes derived from the C2C12 myoblast cell line were induced to undergo cellularization by myoseverin treatment, which destabilizes tubulin filaments. The GSK-3 inhibitor, BIO, was then used to induce dedifferentiation. Induce neuron formation; the cells were incubated with 250 nM reversine for 48 h, and neural induction media (DMEM/F12 supplemented with N2 (Invitrogen)) and 1.5 uM all-trans retinoic acid for 7 days.
C2C12 murine myoblast cell line and 48 h 10 uM BIO treated C2C12 cellulate (derived by 20 M myoseverin treatment for 48 h)
Overall design C2C12 myoblasts were differentiated into myotubes with 2%horse serum in DMEM for 8 days (from 2-4 d, 10 uM AraC treatment was also used to kill any remaining myoblasts). Next, myotubes were cellularized by 20 uM myoseverin treatment for 48 h. 24 h after myoseverin treatment, myotubes were treated with 10 uM BIO for 2d.
Contributor(s) Williams DR, Kim W
Citation(s) 22270490
Submission date Dec 07, 2011
Last update date Jan 12, 2017
Contact name Darren Reece Williams
Phone 8210-8780-0915
Organization name Gwangju Institute of Science and Technology
Department School of Life Sciences
Lab New Drug Targets Laboratory
Street address 1 Oryong-dong
City Gwangju
ZIP/Postal code 500-712
Country South Korea
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (3)
GSM845336 Ex Vivo C2C12 myoblast
GSM845337 Ex Vivo Cellulate 2 days 2.5 uM BIO
GSM845338 Ex Vivo Cellulate 2 days 2.5 uM BIO 7 days neurogenic media
BioProject PRJNA149805

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE34238_RAW.tar 6.6 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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