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| Status |
Public on Mar 12, 2012 |
| Title |
Transcriptional profile of PBMCs in patients with acute RSV or Influenza infection |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To study the transcriptional profile of patients with acute RSV or Influenza infection,children of median age 2.4 months (range 1.5-8.6) hospitalized with acute RSV and influenza virus infection were offered study enrollment after microbiologic confirmation of the diagnosis. Blood samples were collected from them within 42-72 hours of hospitalization. We excluded children with suspected or proven polymicrobial infections, with underlying chronic medical conditions (i.e congenital heart disease, renal insufficiency), with immunodeficiency, or those who received systemic steroids or other immunomodulatory therapies. The RSV cohort consisted of 51 patients with median age of 2 months (range 1.5-3.9) and the influenza cohort had 28 patients with median age of 5.5 months (range 1.4-21). Control samples were obtained from healthy children undergoing elective surgical procedures or at outpatient clinic visits. To exclude viral co-infections we performed nasopharyngeal viral cultures of all subjects. We recruited 10 control patients for the RSV cohort with median age of 6.7 months (range 5-10), and 12 control patients for the influenza cohort with median age of18.5 months (range 10.5-26).
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| Overall design |
We used microarrays to obtain the transcriptional profile of PBMCs from patients with acute RSV or Influenza infection and compared these signatures with the transcriptional profile of primary airway epithelial cells infected with RSV or Influenza.
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| Contributor(s) |
Mejias A, Ioannis I, Flano E |
| Citation(s) |
22398282 |
| |
| Submission date |
Dec 06, 2011 |
| Last update date |
Nov 30, 2020 |
| Contact name |
Damien Chaussabel |
| E-mail(s) |
DChaussabel@benaroyaresearch.org
|
| Organization name |
Baylor Institute for Immunology Research
|
| Street address |
3434 Live Oak
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75204 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (101)
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| This SubSeries is part of SuperSeries: |
| GSE32140 |
The response of PBMCs and primary airway epithelial cells to Influenza and RSV virus |
|
| Relations |
| BioProject |
PRJNA154245 |
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