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| Status |
Public on Oct 16, 2013 |
| Title |
Expression Data from the Riesling grape must fermentation by industrial M2 and M2 nsf1∆ S. cerevisiae strains for functional characterization of the NSF1 (YPL230W) gene via Correlation Clustering-based method |
| Platform organisms |
Schizosaccharomyces pombe; Saccharomyces cerevisiae |
| Sample organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
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| Summary |
The main objectives of this study were to expand our understanding of NSF1 gene function in industrial S. cerevisiae M2 strain during fermentation by finding the largest maximal clique of co-expressed genes (i.e. Interdependent Correlation Cluster), and to establish the impact of Nsf1p on genome-wide gene expression during the fermentation process with possible implications related to wine quality and S. cerevisiae adapation to stressful fermentation conditions
The Affymetrix Yeast 2.0 microarrays were used to capture the global gene expression profile of M2 and M2 nsf1∆ grown under fermentation conditions in Riesling grape must at 18°C with no shaking at various time points. The analysis of this microarray dataset expanded our understanding of the mechanism of action and the roles of NSF1 under fermentation stress conditions.
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| Overall design |
The overall experimental setup consisted of 2 stains (M2 and M2 nsf1∆) and 3 sample time points (24h post-innoculation, 20% and 85% of total glucose fermented) .
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| Contributor(s) |
Bessonov K, van der Merwe G |
| Citation(s) |
24130853 |
| Submission date |
Dec 02, 2011 |
| Last update date |
Feb 21, 2017 |
| Contact name |
Kyrylo Bessonov |
| E-mail(s) |
kbessono@uoguelph.ca
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| Organization name |
University of Guelph
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| Department |
Molecular Biology
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| Lab |
George van der Merwe
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| Street address |
50 Stone Rd East
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| City |
Guelph |
| State/province |
ON |
| ZIP/Postal code |
N1G 2W1 |
| Country |
Canada |
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| Platforms (1) |
| GPL2529 |
[Yeast_2] Affymetrix Yeast Genome 2.0 Array |
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| Samples (14)
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| GSM842092 |
M2 at 24hr, biological rep1 |
| GSM842093 |
M2 at 24hr, biological rep2 |
| GSM842094 |
M2 at 20% total glucose fermented, biological rep1 |
| GSM842095 |
M2 at 20% total glucose fermented, biological rep2 |
| GSM842096 |
M2 at 85% total glucose fermented, biological rep1 |
| GSM842097 |
M2 at 85% total glucose fermented, biological rep2 |
| GSM842098 |
M2 at 85% total glucose fermented, biological rep3 |
| GSM842099 |
M2nsf1∆ mutant at 24hr, biological rep1 |
| GSM842100 |
M2nsf1∆ mutant at 24hr, biological rep2 |
| GSM842101 |
M2nsf1∆ mutant at 20% total glucose fermented, biological rep1 |
| GSM842102 |
M2nsf1∆ mutant at 20% total glucose fermented, biological rep2 |
| GSM842103 |
M2nsf1∆ mutant at 85% total glucose fermented, biological rep1 |
| GSM842104 |
M2nsf1∆ mutant at 85% total glucose fermented, biological rep2 |
| GSM842105 |
M2nsf1∆ mutant at 85% total glucose fermented, biological rep3 |
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| Relations |
| BioProject |
PRJNA149867 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE34117_RAW.tar |
12.3 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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