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| Status |
Public on Oct 04, 2005 |
| Title |
Thyroid hormone induced changes in Xenopus laevis tadpole brain |
| Platform organism |
Xenopus laevis |
| Sample organisms |
Xenopus laevis; unidentified |
| Experiment type |
Expression profiling by array
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| Summary |
Premetamorphic Xenopus laevis tadpoles brain ventricle cells respond to thyroid hormone by proliferation and subsequent differentiation. The goal of this experiment is to identify the genes involved in the TH-induced proliferation pathway in tadpole brain and compare it to TH-induced proliferation and differentiation program in tadpole limb. Xenopus tadpoles (NF54) were treated with 1 mM methimazole in 0.1 X MMR solution for 1 week to block the endogenous TH production and reduce the TH present in the system of the tadpole. They were then treated with 100 nM T3 in 1 mM methimazole and 0.1 x MMR for another 24h and 48h or without T3 for 48h (control group). Brains from the tadpoles were dissected at the end of the experiment.
Keywords: development or differentiation design,organism part comparison design,reference design,replicate design,time series design
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| Overall design |
Premetamorphic Xenopus laevis tadpoles brain ventricle cells respond to thyroid hormone by proliferation and subsequent differentiation. The goal of this experiment is to identify the genes involved in the TH-induced proliferation pathway in tadpole brain and compare it to TH-induced proliferation and differentiation program in tadpole limb. Xenopus tadpoles (NF54) were treated with 1 mM methimazole in 0.1 X MMR solution for 1 week to block the endogenous TH production and reduce the TH present in the system of the tadpole. They were then treated with 100 nM T3 in 1 mM methimazole and 0.1 x MMR for another 24h and 48h or without T3 for 48h (control group). Brains from the tadpoles were dissected at the end of the experiment.
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| Contributor(s) |
Das B, Cai L, Carter MG, Piao Y, Sharov AA, Ko MS, Brown DD |
| Citation(s) |
16458881 |
| |
| Submission date |
Oct 03, 2005 |
| Last update date |
Jan 18, 2013 |
| Contact name |
Minoru S.H. Ko |
| E-mail(s) |
kom@mail.nih.gov
|
| Phone |
410-558-8359
|
| Organization name |
NIH
|
| Department |
National Institute on Aging
|
| Lab |
Lab of Genetics
|
| Street address |
251 Bayview Blvd, Suite 100, 10C
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21224 |
| Country |
USA |
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| Platforms (1) |
| GPL2901 |
Carnegie Xenopus Laevis 22K Microarray v1.0 (60-mer Oligo) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA93535 |
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