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| Status |
Public on Mar 23, 2012 |
| Title |
Time-course HoxB4 ChIP-Seq during HSC development from ES cells |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Efficient in vitro generation of hematopoietic stem cells (HSCs) from embryonic stem cells (ESCs) holds great promise for cell-based therapies of hematological diseases. To date, HoxB4 remains to be the most effective transcription factor (TF) whose over-expression in ESCs confers long-term repopulating ability to ESC-derived HSCs. Despite its importance, the components and dynamics of the HoxB4 transcriptional regulatory network is poorly understood, hindering efforts to develop a more efficient protocol for in vitro derivation of HSCs. Towards this goal, we performed global gene expression profiling and chromatin immunoprecipitation coupled with deep sequencing (ChIP-Seq) at four stages of the HoxB4-mediated HSC development. Joint analyses of ChIP-Seq and gene expression profiles unveil a number of global features of the HoxB4 regulatory network.
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| Overall design |
Cells from three time points of the developmental processes were collected, including days 6, 16, and 26. Deep sequencing were done for both IP and input DNA from cells from each time point.
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| |
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| Contributor(s) |
Tan K |
| Citation(s) |
22438249 |
| Submission date |
Nov 29, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Kai Tan |
| Organization name |
University of California San Diego
|
| Department |
Bioengineering
|
| Street address |
9500 Gilman Drive
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE34014 |
Gene expression profiling and ChIP-Seq study of HoxB4-mediated HSC development from ES cells |
|
| Relations |
| SRA |
SRP009471 |
| BioProject |
PRJNA156455 |
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