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Series GSE33979 Query DataSets for GSE33979
Status Public on Apr 01, 2012
Title Novel roles for Klf1 in regulating the erythroid transcriptome revealed by mRNA-seq
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Klf1 (formerly known as Eklf) regulates the development of erythroid cells from bi-potent progenitor cells via the transcriptional activation of a diverse set of genes. Mice lacking Klf1 die in utero prior to E15 from severe anemia due to the inadequate expression of genes controlling hemoglobin production, cell membrane and cytoskeletal integrity, and the cell cycle and proliferation. We have recently described the full repertoire of Klf1 binding sites in vivo by performing Klf1 ChIP-seq in primary erythroid tissue (E14.5 fetal liver). Here we describe the Klf1-dependent erythroid transcriptome by comparing mRNA-seq from Klf1+/+ and Klf1-/- erythroid tissue. This has revealed novel target genes not previously obtainable by traditional microarray technology and provided novel insights into the function of Klf1 as a transcriptional activator such as interactions with Gata1, Scl/Tal1 and p300. We also describe a set of erythroid specific promoters not previously identified that drive high level expression of otherwise ubiquitously expressed genes in erythroid cells. Additionally, our study has identified for the first time two novel lnc-RNAs that are dynamically expressed during erythroid differentiation as well as a role for Klf1 in directing apoptotic gene expression to drive the terminal stages of erythroid maturation.
Overall design Examination of mRNA expression in 3 Klf1-/- and 3 Klf1+/+ fetal livers

This submission represents mRNA-Seq component of study.
Contributor(s) Tallack MR, Perkins AC
Citation(s) 22835905
Submission date Nov 28, 2011
Last update date May 15, 2019
Contact name Michael Robert Tallack
Phone +61 7 3346 2357
Organization name Institute for Molecular Bioscience, The University of Queensland
Lab Perkins
Street address Services Road
City St Lucia
State/province QLD
ZIP/Postal code 4072
Country Australia
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (6)
GSM839870 WT library 1
GSM839871 WT library 2
GSM839872 WT library 3
SRA SRP009464
BioProject PRJNA150517

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE33979_RAW.tar 4.8 Gb (http)(custom) TAR (of BAM)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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