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| Status |
Public on Dec 13, 2013 |
| Title |
Mechanisms of adaptation to stress and control of virulence in Pseudomonas aeruginosa: the OxyR regulons |
| Platform organism |
Pseudomonas aeruginosa |
| Sample organism |
Pseudomonas aeruginosa PAO1 |
| Experiment type |
Expression profiling by array
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| Summary |
Oxidative stress caused by exposure to reactive oxygen species (ROS), is a major challenge for aerobic and especially anaerobic organisms. Bacteria coordinate the response to oxidative stress through the LysR-type transcriptional regulator (LTTR) OxyR. Extensive studies have focused on the classical Escherichia coli system to shed light on the mode of action of defensive weapons against oxidative stress. The underlying mechanism is mediated via the formation of redox-dependent disulfide bond between two conserved cysteines of OxyR, thus activating transcription of members of the OxyR regulon. However, only fragmentary information on the regulation and function of OxyR has been gleaned through genetic and biochemical analyses in the important opportunistic pathogen P. aeruginosa. In this report, we used a comprehensive transcriptional profiling analysis to delineate the OxyR regulon under three conditions (King’s A medium [Pseudomonas medium or PM], Luria Broth (LB), and LB when oxyR is overexpressed), to investigate its roles in different cellular aspects that are independent of the classical oxidative stress response. Interestingly, when grown in LB, OxyR was found to regulating many genes involved in the process of inter-cellular communication known as quorum sensing (QS). In contrast, when grown in PM, OxyR regulate the expression of a newly identified CSS (cell-surface signaling) system in an OxyR-dependent fashion. In addition, the results from oxyR overexpression further confirmed that OxyR was linked to regulation of QS and Type 3 Secretion (T3SS) in addition to the regulation of antioxidative genes.
Taken together, our results show that, apart from its dominant role in defense against oxidative stress in P. aeruginosa, OxyR acts as a global regulator that provides a link between the regulation of oxidative stress response, QS and virulence.
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| Overall design |
15 samples, representing 5 different biological conditions, including 3 biological replicates for each condition
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| Contributor(s) |
Wei Q, Hildebrand F, Fletcher M, Monsieurs P, Hernalsteens J, Raes J, Ye L, Leys N, Cámara M, Charlier D, Hassett DJ, Cornelis P |
| Citation missing |
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| Submission date |
Nov 22, 2011 |
| Last update date |
Jul 06, 2016 |
| Contact name |
Rob Van Houdt |
| E-mail(s) |
rvhoudto@sckcen.be
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| Phone |
+3214332728
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| Organization name |
SCK-CEN
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| Department |
Interdisciplinary Biosciences
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| Lab |
Microbiology Unit
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| Street address |
Boeretang 200
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| City |
Mol |
| ZIP/Postal code |
2400 |
| Country |
Belgium |
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| Platforms (1) |
| GPL84 |
[Pae_G1a] Affymetrix Pseudomonas aeruginosa Array |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA148359 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE33871_RAW.tar |
9.3 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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